ETFDH (NM_001281738) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ETFQO; MADD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336777 representing NM_001281738. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAAGGTTTGCAGAAGAAGCAGATGTTGTAATAGTTGGTGCAGGCCCTGCAGGGCTCTCTGCAGCT GTTCGTCTAAAACAGTTGGCTGTGGCACATGAAAAGGACATCCGTGTGTGTCTAGTGGAGAAAGCTGCC CAGATAGGAGCTCATACTCTCTCAGGGGCTTGCCTTGATCCAGGTGCTTTTAAAGAACTCTTCCCAGAC TGGAAAGAGAAGGGGGCTCCACTTAACACTCCTGTAACAGAAGACAGATTTGGAATTTTAACAGAGAAA TACAGAATTCCTGTGCCAATTCTTCCAGGGCTTCCAATGAATAATCATGGCAATTACATTGTACGCTTG GGACATTTAGTGAGCTGGATGGGCGAACAAGCAGAAGCCCTTGGTGTTGAAGTATACCCTGGTTATGCA GCTGCTGAGGTCCTTTTTCATGATGATGGTAGTGTAAAAGGAATTGCCACTAACGATGTAGGGATACAA AAGGATGGTGCACCAAAGGCAACATTTGAGAGAGGACTGGAACTACATGCTAAAGTCACAATTTTTGCA GAAGGTTGCCATGGACATCTAGCCAAGCAACTATATAAGAAGTTTGATTTGAGAGCAAATTGTGAACCT CAAACCTACGGGATTGGACTGAAGGAGTTATGGGTTATTGATGAAAAGAACTGGAAACCTGGGAGAGTA GATCACACTGTTGGTTGGCCCTTGGACAGACATACCTATGGAGGATCTTTCCTCTATCATTTGAATGAA GGTGAACCCCTAGTAGCTCTTGGTCTTGTGGTTGGTCTAGACTATCAGAATCCATACCTGAGTCCATTT AGAGAGTTCCAAAGGTGGAAACACCATCCTAGCATTCGGCCAACCTTGGAAGGTGGAAAAAGGATTGCA TACGGAGCCAGAGCTCTCAATGAAGGTGGCTTTCAGTCTATACCAAAACTCACCTTTCCTGGTGGTTTA CTAATTGGTTGTAGTCCTGGTTTTATGAATGTTCCCAAGATCAAAGGTACTCACACAGCAATGAAAAGT GGAATTTTAGCAGCAGAATCTATTTTTAATCAACTAACTAGTGAAAATCTCCAATCAAAGACAATAGGA CTCCATGTAACTGAATATGAGGACAATTTGAAGAACTCATGGGTATGGAAAGAGCTATATTCTGTTAGA AATATAAGACCGTCCTGCCACGGAGTACTGGGTGTATATGGAGGGATGATTTACACTGGAATCTTTTAC TGGATATTGAGAGGAATGGAGCCGTGGACTCTGAAACATAAAGGTTCTGACTTTGAACGGCTCAAGCCA GCCAAGGATTGCACACCTATTGAGTATCCAAAACCCGATGGACAGATCAGTTTTGACCTCTTGTCATCT GTGGCTCTGAGTGGTACTAATCATGAACATGACCAGCCGGCACACTTAACCTTAAGGGATGACAGTATA CCTGTAAATAGAAATCTGTCGATATATGATGGGCCCGAGCAGCGATTCTGTCCTGCAGGAGTTTATGAA TTTGTACCTGTGGAACAAGGTGATGGATTTCGGTTACAGATAAATGCTCAGAACTGTGTACATTGTAAA ACATGTGATATTAAAGATCCAAGTCAGAATATTAACTGGGTGGTACCTGAAGGTGGAGGAGGACCTGCT TACAATGGAATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281738
Insert Size	1671 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281738.1
RefSeq Size	1952 bp
RefSeq ORF	1671 bp
Locus ID	2110
MW	61.4 kDa
Gene Summary	This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) represents use of an alternate promoter, differs in the 5' UTR, and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1.

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