NADPH oxidase 4 (NOX4) (NM_001291929) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KOX; KOX-1; RENOX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336766 representing NM_001291929. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTTCTGCAGTTACTGATTTAGGTTCCATTGAAAGCACTGTGTCGAAGAATTTACCTGTGGTTTCT ATTCATCAGCTAGGATTGTGTCTAAGCAGAGCCTCAGCATCTGTTCTTAACCTCAACTGCAGCCTTATC CTTTTACCCATGTGCCGAACACTCTTGGCTTACCTCCGAGGATCACAGAAGGTTCCAAGCAGGAGAACC AGGAGATTGTTGGATAAAAGCAGAACATTCCATATTACCTGTGGTGTTACTATCTGTATTTTCTCAGGC GTGCATGTGGCTGCCCATCTGGTGAATGCCCTCAACTTCTCAGTGAATTACAGTGAAGACTTTGTTGAA CTGAATGCAGCAAGATACCGAGATGAGGATCCTAGAAAACTTCTCTTCACAACTGTTCCTGGCCTGACA GGGGTCTGCATGGTGGTGGTGCTATTCCTCATGATCACAGCCTCTACATATGCAATAAGAGTTTCTAAC TATGATATCTTCTGGTATACTCATAACCTCTTCTTTGTCTTCTACATGCTGCTGACGTTGCATGTTTCA GGAGGGCTGCTGAAGTATCAAACTAATTTAGATACCCACCCTCCCGGCTGCATCAGTCTTAACCGAACC AGCTCTCAGAATATTTCCTTACCAGAGTATTTCTCAGAACATTTTCATGAACCTTTCCCTGAAGGATTT TCAAAACCGGCAGAGTTTACCCAGCACAAATTTGTGAAGATTTGTATGGAAGAGCCCAGATTCCAAGCT AATTTTCCACAGACTTGGCTTTGGATTTCTGGACCTTTGTGCCTGTACTGTGCCGAAAGACTTTACAGG TATATCCGGAGCAATAAGCCAGTCACCATCATTTCGGTCATAAGTCATCCCTCAGATGTCATGGAAATC CGAATGGTCAAAGAAAATTTTAAAGCAAGACCTGGTCAGTATATTACTCTACATTGTCCCAGTGTATCT GCATTAGAAAATCATCCATTTACCCTCACAATGTGTCCAACTGAAACCAAAGCAACATTTGGGGTTCAT CTTAAAATAGTAGGAGACTGGACAGAACGATTTCGAGATTTACTACTGCCTCCATCTAGTCAAGACTCC GAAATTCTGCCCTTCATTCAATCTAGAAATTATCCCAAGCTGTATATTGATGGTCCTTTTGGAAGTCCA TTTGAGGAATCACTGAACTATGAGGTCAGCCTCTGCGTGGCTGGAGGCATTGGAGTAACTCCATTTGCA TCAATACTCAACACCCTGTTGGATGACTGGAAACCATACAAGCTTAGAAGACTATACTTTATTTGGGTA TGCAGAGATATCCAGTCCTTCCGTTGGTTTGCAGATTTACTCTGTATGTTGCATAACAAGTTTTGGCAA GAGAACAGACCTGACTATGTCAACATCCAGCTGTACCTCAGTCAAACAGATGGGATACAGAAGATAATT GGAGAAAAATATCATGCACTGAATTCAAGACTGTTTATAGGACGTCCTCGGTGGAAACTTTTGTTTGAT GAAATAGCAAAATATAACAGAGGAAAAACAGTTGGTGTTTTCTGTTGTGGACCCAATTCACTATCCAAG ACTCTTCATAAACTGAGTAACCAGAACAACTCATATGGGACAAGATTTGAATACAATAAAGAGTCTTTC AGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291929
Insert Size	1662 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291929.1
RefSeq Size	4122 bp
RefSeq ORF	1662 bp
Locus ID	50507
Protein Families	Druggable Genome, Transmembrane
MW	63.5 kDa
Gene Summary	This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009] Transcript Variant: This variant (6) differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (f) has a shorter and distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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