Trehalase (TREH) (NM_001301065) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TRE; TREA; TREHD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336763 representing NM_001301065. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCAGGGAGGACCTGGGAGCTGTGCCTGCTACTGCTGCTGGGGCTGGGACTGGGGTCCCAGGAGGCC CTACCCCCACCCTGTGAGAGTGAGATTTACTGCCACGGGGAGCTCCTAAACCAAGTTCAAATGGCCAAG CTCTACCAGGATGACAAGCAGTTTGTGGACATGCCACTGTCTATAGCTCCAGAACAAGTCCTGCAGACC TTCACTGAGCTGTCCAGGGACCACAATCACAGCATCCCCAGGGAGCAGCTGCAGGCGTTTGTCCACGAA CACTTCCAGGCCAAGGGGCAGGAGCTGCAGCCCTGGACCCCTGCAGACTGGAAAGACAGCCCCCAGTTC CTGCAGAAGATTTCAGATGCCAAACTGCGTGCCTGGGCAGGGCAGCTGCATCAGCTCTGGAAGAAGCTG GGGAAGAAGATGAAGCCAGAGGTTCTCAGCCACCCTGAGCGGTTCTCTCTCATCTACTCAGAACATCCC TTCATTGTGCCTGGCGGTCGCTTTGTTGAGTTCTACTACTGCTATGGGCATGTCCCCAATGGTGGGCGC GTGTACTACCTGCAGCGGAGCCAGCCCCCACTCTTGACCCTCATGATGGATTGCTACTTGACTCACACC AATGACACCGCCTTTCTACAGGAAAACATTGAAACACTAGCCTTGGAATTGGACTTTTGGACCAAGAAC AGGACTGTCTCTGTGAGCTTGGAGGGAAAGAACTACCTCCTGAATCGCTATTATGTCCCTTATGGGGGA CCCAGGCCTGAGTCCTACAGCAAAGATGTGGAGTTGGCTGACACCTTGCCAGAAGGAGACCGGGAGGCT CTGTGGGCTGAGCTCAAGGCTGGGGCTGAGTCTGGCTGGGACTTCTCTTCACGCTGGCTCATTGGAGGC CCAAACCCCAACTCGCTTAGCGGCATCCGAACAAGCAAACTGGTGCCTGTTGACCTGAATGCCTTCCTA TGCCAAGCAGAGGAGCTGATGAGCAACTTCTATTCCAGGCTGGGGAACGACTCCCAGGCCACGAAGTAC AGAATCCTGCGGTCGCAGCGCTTGGCCGCCCTGAACACAGTCCTGTGGGATGAGCAGACCGGAGCCTGG TTCGATTACGACCTTGAGAAGAAGAAGAAAAACCGGGAGTTTTACCCATCCAACCTCACTCCACTCTGG GCCGGGTGTTTCTCTGACCCTGGCGTGGCGGACAAGGCTCTGAAATACCTGGAGGACAACCGGATCCTG ACTTACCAGTATGGGATCCCGACCTCTCTCCAGAAGACAGGCCAGCAGTGGGATTTCCCCAATGCCTGG GCCCCCCTGCAGGACCTGGTCATCAGAGGCCTGGCCAAGGCACCTTTACGTCGGGCCCAGGAAGTGGCT TTCCAGCTGGCTCAGAATTGGATCCGAACCAATTTTGATGTCTACTCGCAGAAGTCAGCCATGTATGAG AAGTATGACGTCAGCAACGGTGGACAGCCCGGTGGGGGAGGAGAATATGAAGTTCAGGAGGGATTTGGC TGGACGAATGGCGTGGTCCTGATGCTGCTGGACCGCTATGGTGACCGGCTGACCTCAGGGGCCAAGCTG GCTTTCCTGGAGCCCCACTGCCTGGCGGCCACCCTTCTGCCCAGCCTCCTGCTCAGCCTCCTGCCATGG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301065
Insert Size	1659 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301065.1
RefSeq Size	1781 bp
RefSeq ORF	1659 bp
Locus ID	11181
UniProt ID	O43280
Protein Pathways	Starch and sucrose metabolism
MW	62.9 kDa
Gene Summary	This gene encodes an enzyme that hydrolyses trehalose, a disaccharide formed from two glucose molecules found mainly in fungi, plants, and insects. A partial duplication of this gene is located adjacent to this locus on chromosome 11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

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