2 Hydroxy phytanoyl CoA lyase (HACL1) (NM_001284413) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2-HPCL; HPCL; HPCL2; PHYH2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336755 representing NM_001284413. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCGGACAGTAACTTCGCAGAGCGCAGCGAGGAGCAGGTGTCTGGTGCTAAAGTCATCGCTCAGGCC CTGAAAACGCAAGATGTGGAGTACATATTTGGCATCGTAGGCATCCCAGTGACCGAAATCGCCATTGCT GCCCAGCAGCTAGGCATCAAGTACATCGGGATGAGGAATGAGCAAGCGGCTTGTTATGCTGCCTCCGCG ATTGGATATCTGACAAGCAGGCCCTTGCTTGTGATTGGTGGTTCCTCTGAAAGAAACCAAGAAACAATG GGAGCTTTCCAGGAGTTTCCTCAGGTTGAAGCTTGTAGATTATATACCAAGTTCTCTGCCCGCCCAAGC AGCATAGAAGCTATTCCTTTTGTTATTGAAAAGGCAGTGAGAAGCAGTATCTATGGTCGTCCAGGTGCT TGCTATGTTGACATACCAGCAGATTTTGTGAACCTTCAGGTGAATGTGAATTCTATAAAGTACATGGAA CGCTGCATGTCACCTCCTATTAGCATGGCAGAAACCTCTGCTGTGTGCACGGCGGCTTCTGTTATTAGG AATGCCAAACAACCCCTTCTTATCATCGGGAAAGGTGCTGCTTACGCTCATGCAGAAGAGAGTATCAAG AAATTGGTGGAGCAATATAAACTGCCATTTTTGCCCACCCCTATGGGGAAGGGTGTTGTCCCTGACAAC CATCCATACTGTGTAGGTGCAGCCAGATCCAGGGCTTTGCAATTTGCTGATGTAATTGTGTTATTTGGT GCCAGACTAAATTGGATTTTACATTTTGGACTGCCTCCAAGATATCAGCCAGATGTGAAGTTTATCCAG GTTGATATCTGTGCAGAAGAATTGGGGAATAATGTAAAGCCCGCTGTTACTTTGCTAGGAAACATACAT GCTGTCACTAAGCAGCTTTTAGAGGAACTTGATAAAACACCATGGCAGTATCCTCCAGAGAGCAAGTGG TGGAAAACTCTGAGAGAAAAAATGAAGAGCAATGAAGCTGCATCCAAGGAACTAGCTTCTAAAAAATCC CTGCCTATGAATTATTACACAGTATTCTACCATGTTCAAGAACAACTACCTAGAGACTGTTTCGTGGTA AGTGAAGGAGCAAATACTATGGACATTGGACGGACTGTGCTTCAGAACTACCTTCCTCGTCACAGGCTT GATGCTGGTACTTTCGGAACAATGGGAGTTGGTTTGGGATTTGCTATTGCAGCTGCCGTGGTGGCTAAA GATAGAAGCCCTGGGCAATGGATCATCTGTGTGGAAGGAGACAGTGCATTTGGGTTTTCTGGCATGGAG GTAGAAACCATCTGCAGGTACAACTTGCCAATCATACTGTTGGTAGTGAATAACAATGGAATTTACCAA GGTTTTGATACAGATACTTGGAAAGAAATGTTAAAATTTCAAGATGCTACTGCAGTGGTCCCTCCAATG TGTTTGCTGCCAAATTCACATTATGAGCAAGTCATGACTGCATTTGGAGGCAAAGGGTATTTTGTACAA ACACCAGAAGAACTCCAAAAATCCCTGAGGCAGAGCCTAGCAGACACAACTAAACCTTCTCTTATCAAC ATCATGATTGAGCCACAAGCCACACGGAAGGCCCAGGATTTTCATTGGCTGACCCGCTCTAATATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001284413
Insert Size	1656 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284413.1
RefSeq Size	2206 bp
RefSeq ORF	1656 bp
Locus ID	26061
UniProt ID	Q9UJ83
MW	61.1 kDa
Gene Summary	Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

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