EHD1 (NM_001282445) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H-PAST; HPAST1; PAST; PAST1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336741 representing NM_001282445. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAACAGCCAGGAACTGCAGCCAGCCCCGTGTCCGGCAGTATGTTCAGCTGGGTCAGCAAGGATGCC CGCCGCAAGAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAGGGGCTGCGGCAGCTGTACGCGCAGAAG CTGCTACCCCTGGAGGAGCACTACCGCTTCCACGAGTTCCACTCGCCCGCGCTGGAGGACGCTGACTTC GACAACAAGCCTATGGTGCTCCTCGTGGGGCAGTACAGCACGGGCAAGACCACCTTCATCCGACACCTG ATCGAGCAGGACTTCCCGGGGATGCGCATCGGGCCCGAGCCCACCACCGACTCCTTCATCGCCGTCATG CACGGCCCCACTGAGGGCGTGGTGCCGGGCAACGCGCTCGTGGTGGACCCGCGGCGCCCCTTCCGCAAG CTCAACGCGTTTGGCAACGCTTTCCTCAACAGGTTCATGTGTGCCCAGCTGCCCAACCCCGTCCTGGAC AGCATCAGCATCATCGACACCCCCGGGATCCTGTCTGGAGAGAAGCAGCGGATCAGCAGAGGCTATGAC TTTGCAGCCGTCCTGGAGTGGTTCGCGGAGCGTGTGGACCGCATCATCCTGCTCTTCGACGCCCACAAG CTGGACATCTCCGATGAGTTCTCGGAAGTGATCAAGGCTCTGAAGAACCATGAGGACAAGATCCGCGTG GTGCTGAACAAGGCAGACCAGATCGAGACGCAGCAGCTGATGCGGGTGTACGGGGCCCTCATGTGGTCC CTGGGCAAGATCATCAACACCCCCGAGGTGGTCAGGGTCTACATCGGCTCCTTCTGGTCCCACCCGCTC CTCATCCCCGACAACCGCAAGCTCTTTGAGGCCGAGGAGCAGGACCTCTTCAAGGACATCCAGTCACTG CCCCGAAACGCCGCCCTCAGGAAGCTCAATGACCTGATCAAGCGGGCACGGCTGGCCAAGGTTCACGCC TACATCATCAGCTCCCTCAAGAAAGAGATGCCCAATGTCTTTGGTAAAGAGAGCAAAAAGAAAGAGCTG GTGAACAACCTGGGAGAGATCTACCAGAAGATTGAGCGCGAGCACCAGATCTCCCCTGGGGACTTCCCG AGCCTCCGCAAGATGCAGGAACTCCTGCAGACCCAGGACTTCAGCAAGTTCCAGGCGCTGAAGCCCAAG CTGCTGGACACGGTGGATGACATGCTGGCCAACGACATCGCGCGGCTGATGGTGATGGTGCGGCAGGAG GAGTCCCTGATGCCTTCCCAGGTGGTCAAGGGCGGCGCCTTTGACGGCACCATGAACGGGCCGTTCGGG CACGGCTACGGCGAGGGGGCCGGCGAGGGCATCGACGACGTGGAGTGGGTGGTGGGCAAGGACAAGCCC ACCTACGACGAGATCTTCTACACGCTGTCCCCTGTCAACGGCAAGATCACGGGCGCCAACGCCAAGAAG GAGATGGTGAAGTCCAAGCTCCCCAACACCGTGCTAGGGAAGATCTGGAAGCTGGCCGACGTGGACAAG GACGGGCTGCTGGACGACGAGGAGTTCGCGCTGGCCAACCACCTCATCAAGGTCAAGCTGGAGGGCCAC GAGCTGCCCGCCGACCTGCCCCCGCACCTGGTGCCGCCCTCCAAGCGCAGACATGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282445
Insert Size	1647 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282445.1
RefSeq Size	3354 bp
RefSeq ORF	1647 bp
Locus ID	10938
UniProt ID	Q9H4M9
Protein Families	Druggable Genome
Protein Pathways	Endocytosis
MW	61.9 kDa
Gene Summary	This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (3) lacks an internal exon in the 5' region, which results in an upstream in-frame AUG start codon, compared to variant 1. The resulting isoform (2) has a longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.