GPR161 (NM_001267611) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RE2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336733 representing NM_001267611. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAAGTCGTCCAGCATGCTCTGCCCACCCCACGCCGAGGTGCACTGACCATGAGCCTCAACTCCTCC CTCAGCTGCAGGAAGGAGCTGAGTAATCTCACTGAGGAGGAGGGTGGCGAAGGGGGCGTCATCATCACC CAGTTCATCGCCATCATTGTCATCACCATTTTTGTCTGCCTGGGAAACCTGGTCATCGTGGTCACCTTG TACAAGAAGTCCTACCTCCTCACCCTCAGCAACAAGTTCGTCTTCAGCCTGACTCTGTCCAACTTCCTG CTGTCCGTGTTGGTGCTGCCTTTTGTGGTGACGAGCTCCATCCGCAGGGAATGGATCTTTGGTGTAGTG TGGTGCAACTTCTCTGCCCTCCTCTACCTGCTGATCAGCTCTGCCAGCATGCTAACCCTCGGGGTCATT GCCATCGACCGCTACTATGCTGTCCTGTACCCCATGGTGTACCCCATGAAGATCACAGGGAACCGGGCT GTGATGGCACTTGTCTACATCTGGCTTCACTCGCTCATCGGCTGCCTGCCACCCCTGTTTGGTTGGTCA TCCGTGGAGTTTGACGAGTTCAAATGGATGTGTGTGGCTGCTTGGCACCGGGAGCCTGGCTACACGGCC TTCTGGCAGATCTGGTGTGCCCTCTTCCCCTTTCTGGTCATGCTGGTGTGCTATGGCTTCATCTTCCGC GTGGCCAGGGTCAAGGCACGCAAGGTGCACTGTGGCACAGTCGTCATCGTGGAGGAGGATGCTCAGAGG ACCGGGAGGAAGAACTCCAGCACCTCCACCTCCTCTTCAGGCAGCAGGAGGAATGCCTTTCAGGGTGTG GTCTACTCGGCCAACCAGTGCAAAGCCCTCATCACCATCCTGGTGGTCCTCGGTGCCTTCATGGTCACC TGGGGCCCCTACATGGTTGTCATCGCCTCTGAGGCCCTCTGGGGGAAAAGCTCCGTCTCCCCGAGCCTG GAGACTTGGGCCACATGGCTGTCCTTTGCCAGCGCTGTCTGCCACCCCCTGATCTATGGACTCTGGAAC AAGACAGTTCGCAAAGAACTACTGGGCATGTGCTTTGGGGACCGGTATTATCGGGAACCATTTGTGCAA CGACAGAGGACTTCCAGGCTCTTCAGCATTTCCAACAGGATCACAGACCTGGGCCTGTCCCCACACCTC ACTGCGCTCATGGCAGGTGGACAGCCCCTGGGGCACAGCAGCAGCACGGGGGACACTGGCTTCAGCTGC TCCCAGGACTCAGGGACAGATATGATGCTGCTTGAGGACTACACGTCTGATGACAACCCTCCCTCTCAC TGCACTTGCCCACCCAAGAGAAGGAGCTCGGTGACATTTGAGGATGAAGTGGAACAAATCAAAGAAGCT GCCAAGAACTCGATTCTTCATGTGAAAGCTGAAGTACACAAGTCCTTGGACAGTTACGCAGCAAGCTTG GCCAAAGCCATTGAGGCCGAAGCCAAAATCAACTTATTTGGGGAGGAGGCTTTGCCAGGGGTCTTGGTT ACAGCACGGACTGTCCCGGGGGGCGGCTTCGGGGGCCGCCGAGGCAGCAGAACTCTTGTGAGCCAGAGG CTGCAGTTGCAGAGCATCGAAGAAGGAGATGTTTTAGCTGCCGAGCAGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001267611
Insert Size	1641 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267611.1
RefSeq Size	7815 bp
RefSeq ORF	1641 bp
Locus ID	23432
Protein Families	Druggable Genome, GPCR, Transmembrane
MW	60.4 kDa
Gene Summary	The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. This gene is overexpressed in triple-negative breast cancer, and disruption of this gene slows the proliferation of basal breast cancer cells. Therefore, this gene is a potential drug target for triple-negative breast cancer. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (4) lacks a portion of the 5' coding region, and initiates translation at an upstream start codon, compared to variant 1. The resulting isoform (3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.