BTD (NM_001281723) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336727 representing NM_001281723. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCAGAGGGAGGCGGGACTAGCAGGAGATTGCTGCCTATGCAAAGCAGATTTGTGGTCTGCATTATG TCTGGAGCCAGAAGTAAGCTTGCTCTTTTCCTCTGCGGCTGTTACGTGGTTGCCCTGGGAGCCCACACC GGGGAGGAGAGCGTGGCTGACCATCACGAGGCTGAATATTATGTGGCTGCCGTGTATGAGCATCCATCC ATCCTGAGTCTGAACCCTCTGGCTCTCATCAGCCGCCAAGAGGCCTTGGAGCTCATGAACCAGAACCTT GACATCTATGAACAGCAAGTGATGACTGCAGCCCAAAAGGATGTACAGATTATAGTGTTTCCAGAAGAT GGCATTCATGGATTCAACTTTACAAGAACATCCATTTATCCATTTTTGGACTTCATGCCGTCTCCCCAG GTGGTCAGGTGGAACCCATGCCTGGAGCCTCACCGCTTCAATGACACAGAGGTGCTCCAGCGCCTGAGT TGTATGGCCATCAGGGGAGATATGTTCTTGGTGGCCAATCTTGGGACAAAGGAGCCTTGTCATAGCAGT GACCCAAGGTGCCCAAAAGATGGGAGATACCAGTTCAACACAAATGTCGTGTTCAGCAATAATGGAACC CTTGTTGACCGCTACCGTAAACACAACCTCTACTTTGAGGCAGCATTCGATGTTCCTCTTAAAGTGGAT CTCATCACCTTTGATACCCCCTTTGCTGGCAGGTTTGGCATCTTCACATGCTTTGATATATTGTTCTTT GACCCTGCCATCAGAGTCCTCAGAGACTACAAGGTGAAGCATGTTGTGTACCCAACTGCCTGGATGAAC CAGCTCCCACTCTTGGCAGCAATTGAGATTCAGAAAGCTTTTGCTGTTGCCTTTGGCATCAACGTTCTG GCAGCTAATGTCCACCACCCAGTTCTGGGGATGACAGGAAGTGGCATACACACCCCTCTGGAGTCCTTT TGGTACCATGACATGGAAAATCCCAAAAGTCACCTTATAATTGCCCAGGTGGCCAAAAATCCAGTGGGT CTCATTGGTGCAGAGAATGCAACAGGTGAAACGGACCCATCCCATAGTAAGTTTTTAAAAATTTTGTCA GGCGATCCGTACTGTGAGAAGGATGCTCAGGAAGTCCACTGTGATGAGGCCACCAAGTGGAACGTGAAT GCTCCTCCCACATTTCACTCTGAGATGATGTATGACAATTTCACCCTGGTCCCTGTCTGGGGAAAGGAA GGCTATCTCCACGTCTGTTCCAATGGCCTCTGCTGTTATTTACTTTACGAGAGGCCCACCTTATCCAAA GAGCTGTATGCCCTGGGGGTCTTTGATGGGCTTCACACAGTACATGGCACTTACTACATCCAAGTGTGT GCCCTGGTCAGGTGTGGGGGTCTTGGCTTCGACACCTGTGGACAGGAAATCACAGAGGCCACGGGGATA TTTGAGTTTCACCTGTGGGGCAACTTCAGTACTTCCTATATCTTTCCTTTGTTTCTGACCTCAGGGATG ACCCTAGAAGTCCCTGACCAGCTTGGCTGGGAGAATGACCACTATTTCCTGAGGAAAAGTAGGCTGTCC TCTGGGCTGGTGACGGCGGCTCTCTATGGGCGCTTGTATGAGAGGGACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281723
Insert Size	1638 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281723.2
RefSeq Size	4169 bp
RefSeq ORF	1638 bp
Locus ID	686
UniProt ID	P43251
Protein Families	Druggable Genome, Secreted Protein
Protein Pathways	Biotin metabolism, Metabolic pathways
MW	61.3 kDa
Gene Summary	The protein encoded by this gene functions to recycle protein-bound biotin by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. The encoded protein has also been shown to have biotinyl transferase activity. Mutations in this gene are associated with biotinidase deficiency. Multiple transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (1) encodes isoform 1. Variants 1-4 and 6 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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