ABLIM3 (NM_001301028) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HMFN1661
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336722 representing NM_001301028. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAACACTAGCATTCCTTATCAGCAGAATCCTTACAATCCACGGGGCAGCTCCAATGTCATCCAGTGC TACCGCTGTGGAGACACCTGCAAAGGGGAAGTGGTCCGCGTGCACAACAACCACTTCCACATCAGATGC TTCACCTGTCAAGTATGTGGCTGTGGCCTGGCCCAGTCAGGCTTCTTCTTCAAGAACCAGGAGTACATC TGCACCCAGGACTACCAGCAACTCTATGGCACCCGCTGTGACAGCTGCCGGGACTTCATCACAGGCGAA GTCATCTCGGCCCTGGGCCGCACTTACCACCCCAAGTGCTTCGTGTGCAGCTTGTGCAGGAAGCCTTTC CCCATTGGAGACAAGGTGACCTTCAGCGGTAAAGAATGTGTGTGCCAAACGTGCTCCCAGTCCATGGCC AGCAGTAAGCCCATCAAGATTCGTGGACCAAGCCACTGTGCCGGGTGCAAGGAGGAGATCAAGCACGGC CAGTCACTCCTGGCTCTGGACAAGCAGTGGCACGTCAGCTGCTTCAAGTGCCAGACCTGCAGCGTCATC CTCACCGGGGAGTATATCAGCAAGGATGGTGTTCCATACTGTGAGTCCGACTACCATGCCCAGTTTGGC ATTAAATGTGAGACTTGTGACCGATACATCAGTGGCAGAGTCTTGGAGGCAGGAGGGAAGCACTACCAC CCAACCTGTGCCAGGTGTGTACGCTGCCACCAGATGTTCACCGAAGGAGAGGAAATGTACCTCACAGGT TCCGAGGTTTGGCACCCCATCTGCAAACAGGCAGCCCGGGCAGAGAAGAAGTTAAAGCATAGACGGACA TCTGAAACCTCCATCTCACCCCCTGGATCCAGCATTGGGTCACCCAACCGAGTCATCTGCGACATCTAC GAGAACCTGGACCTCCGGCAGAGACGGGCCTCCAGCCCGGGGTACATAGACTCCCCCACCTACAGCCGG CAGGGCATGTCCCCCACCTTCTCCCGCTCACCTCACCACTACTACCGCTCTGGTGATTTGTCTACAGCA ACCAAGAGCAAAACAAGTGAAGACATCAGCCAGACCTCCAAGTACAGTCCCATCTACTCGCCAGACCCC TACTATGCTTCGGAGTCTGAGTACTGGACCTACCATGGGTCCCCCAAAGTGCCCCGAGCCAGAAGGTTC TCGTCTGGAGGAGAGGAGGATGATTTTGACCGCAGCATGCACAAGCTCCAAAGTGGAATTGGCCGGCTG ATTCTGAAGGAAGAAATGAAGGCCCGGTCGAGCTCCTATGCAGATCCCTGGACCCCTCCCCGGAGCTCC ACCAGCAGCCGGGAAGCCCTGCACACAGCTGGCTATGAGATGTCCCTCAATGGCTCCCCTCGGTCGCAC TACCTGGCTGACAGTGATCCTCTCATCTCCAAATCTGCCTCCCTGCCTGCCTACCGAAGAAATGGGCTG CACAGGACACCCAGCGCAGACCTCTTCCACTACGACAGCATGAACGCAGTCAACTGGGGCATGCGAGAG TACAAGATCTACCCTTATGAACTGCTGCTGGTGACTACAAGAGGAAGAAACCGACTGCCCAAGGATGTA GACAGGACCCGTTTAGAGGGAAACTTTTGGAAGAGTGGCTGCTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301028
Insert Size	1635 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301028.1
RefSeq Size	3131 bp
RefSeq ORF	1635 bp
Locus ID	22885
UniProt ID	O94929
Protein Pathways	Axon guidance
MW	61.6 kDa
Gene Summary	This gene encodes a member of the actin-binding LIM (abLIM) family of proteins. These proteins are characterized by an N-terminal LIM domain and a C-terminal dematin-like domain. The encoded protein interacts with actin filaments and may be a component of adherens junctions in several cell types. A variant of this gene may be associated with pain sensitivity in male human patients. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (5) lacks several in-frame exons and has an alternate splice site in the 3' coding region, compared to variant 1. The resulting isoform (4) lacks two internal segments and has a shorter and distinct C-terminus, compared to isoform 1.

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