APPBP1 (NAE1) (NM_001286500) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	A-116A10.1; APPBP1; HPP1; ula-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336682 representing NM_001286500. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCAGCTGGGAAAGCTGCTCAAGGAGCAGAAGTACGACCGGCAGCTGAGGTTGTGGGGTGATCAT GGGCAAGAGGCTTTAGAATCTGCTCATGTTTGCCTAATAAATGCAACAGCCACAGGAACTGAAATTCTT AAAAACTTGGTACTACCAGGTAACGTAGGTATTGGTTCGTTTACAATTATTGATGGAAATCAGGTCAGC GGAGAAGATGCTGGAAACAATTTCTTCCTTCAAAGAAGCAGTATCGGCAAGAACCGAGCTGAAGCTGCC ATGGAATTCTTACAAGAATTAAATAGCGATGTCTCTGGAAGTTTTGTGGAAGAGAGTCCAGAAAACCTT CTAGACAATGATCCCTCATTTTTCTGTAGGTTTACTGTTGTAGTTGCAACTCAGCTTCCTGAAAGCACT TCACTACGCTTAGCAGATGTCCTCTGGAATTCCCAGATTCCTCTTTTGATCTGTAGGACATATGGACTA GTTGGTTATATGAGGATCATTATAAAAGAACATCCAGTAATAGAATCTCATCCAGATAATGCATTAGAG GATCTACGACTAGATAAGCCATTTCCTGAACTGAGAGAACATTTTCAGTCCTATGATTTGGATCATATG GAAAAAAAGGACCACAGTCATACTCCATGGATTGTGATCATAGCTAAATATTTAGCACAGTGGTATAGT GAAACAAATGGACGAATACCTAAAACGTATAAAGAAAAAGAGGACTTCAGAGATTTGATTAGACAAGGA ATTCTAAAAAATGAAAATGGGGCTCCAGAAGATGAAGAGAATTTTGAAGAAGCTATTAAAAATGTGAAC ACAGCACTAAATACAACTCAGATCCCAAGCAGTATTGAAGATATATTTAATGATGATCGCTGCATAAAT ATCACCAAACAGACTCCATCATTTTGGATTTTAGCTCGTGCCTTAAAGGAATTTGTGGCCAAAGAGGGT CAAGGAAATTTACCTGTTCGAGGCACAATTCCTGATATGATTGCAGATTCAGGCAAATATATAAAACTG CAAAACGTTTACCGTGAAAAAGCAAAGAAAGATGCTGCCGCTGTGGGTAATCATGTTGCCAAATTGCTG CAGTCCATTGGCCAGGCACCAGAGTCCATTTCAGAGAAAGAATTAAAATTACTCTGCAGCAATTCTGCA TTTCTTCGAGTGGTAAGATGTCGATCCTTAGCTGAAGAATATGGTTTGGATACAATTAACAAGGATGAA ATTATTTCTAGCATGGACAATCCAGATAATGAAATAGTGTTGTACTTAATGTTACGGGCTGTTGATAGA TTTCATAAACAACAGGGTAGATATCCAGGAGTATCTAACTATCAAGTTGAAGAAGATATAGGAAAGTTG AAGTCTTGTCTCACTGGCTTCCTTCAGGAATATGGTTTATCTGTAATGGTGAAAGATGATTATGTCCAC GAATTTTGCCGATATGGAGCTGCTGAGCCACATACCATTGCTGCATTCTTGGGGGGAGCTGCTGCTCAA GAGGTCATCAAAATAATCACCAAACAATTTGTAATTTTTAATAATACTTACATTTACAGTGGCATGTCA CAAACTTCAGCAACTTTCCAGTTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286500
Insert Size	1614 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286500.1
RefSeq Size	1826 bp
RefSeq ORF	1614 bp
Locus ID	8883
UniProt ID	Q13564
Protein Pathways	Alzheimer's disease
MW	60.5 kDa
Gene Summary	The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (4) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (d) has the same N- and C-termini but contains an additional 3 aa compared to variant 1.

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