TTC8 (NM_001288781) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BBS8; RP51
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336637 representing NM_001288781. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCTCGGAGATGGAGCCGCTGCTCCTGGCCTGGAGCTATTTTAGGCGCAGGAAGTTCCAGCTCTGC GCCGATCTATGCACGCAGATGCTGGAGAAGTCCCCTTATGACCAGGCAGCTTGGATCTTAAAAGCAAGA GCGCTAACAGAAATGGTATACATAGATGAAATTGATGTAGATCAGGAAGGAATTGCAGAAATGATGCTG GATGAAAATGCTATAGCTCAAGTTCCACGCCCTGGAACGTCTTTGAAACTCCCTGGAACTAATCAGACA GGAGGGCCTAGCCAGGCCGTTAGGCCAATCACACAAGCTGGAAGACCCATTACAGGTTTCCTCAGGCCC AGCACGCAGAGTGGAAGGCCAGGCACTATGGAACAGGCTATCAGAACACCCAGAACCGCCTACACAGCC CGCCCTATCACCAGCTCCTCCGGAAGATTTGTCAGGCTGGGAACGGCTTCCATGCTTACAAGTCCTGAT GGACCATTTATAAATTTATCTAGGCTGAATTTAACAAAGTATTCCCAGAAACCTAAGTTGGCAAAGGCT TTGTTTGAGTATATCTTTCATCATGAAAATGATGTTAAGACTATTCATCTTGAAGATGTAGTTCTACAT CTTGGAATTTACCCATTCTTATTGAGGAATAAAAATCACATTGAAAAAAATGCTTTGGATCTGGCTGCC CTCTCCACAGAACATTCTCAGTACAAGGACTGGTGGTGGAAAGTACAGATTGGAAAATGTTACTACAGG TTGGGAATGTATCGTGAAGCAGAAAAACAGTTTAAATCAGCCCTGAAGCAGCAGGAAATGGTAGATACA TTTCTGTACTTGGCAAAAGTTTATGTCTCATTGGATCAACCTGTGACTGCTTTAAATCTTTTCAAACAA GGCTTAGATAAGTTTCCAGGAGAAGTAACCCTGCTCTGTGGAATTGCAAGAATCTATGAGGAAATGAAC AATATGTCATCAGCAGCAGAATATTACAAAGAAGTTTTGAAACAAGACAATACTCATGTGGAAGCCATC GCATGCATTGGAAGCAACCACTTCTATTCTGATCAGCCAGAAATAGCTCTCCGGTTTTACAGGCGGCTG CTGCAGATGGGCATTTATAACGGCCAGCTTTTTAACAATCTGGGGCTGTGTTGCTTCTATGCCCAGCAG TATGATATGACTCTGACCTCATTTGAACGTGCCCTTTCTTTGGCTGAAAATGAAGAAGAGGCAGCTGAT GTCTGGTACAACTTGGGACATGTAGCTGTGGGAATAGGAGATACAAATTTGGCCCATCAGTGCTTCAGG CTGGCTCTGGTCAACAACAACAACCACGCCGAGGCCTACAACAACCTGGCTGTGCTGGAGATGCGGAAG GGCCACGTTGAACAGGCAAGGGCACTATTACAAACTGCATCATCATTAGCACCCCATATGTATGAACCG CATTTTAATTTTGCAACAATCTCTGATAAGATTGGAGATCTGCAGAGAAGCTATGTTGCTGCGCAGAAG TCTGAAGCAGCATTTCCAGACCATGTGGACACACAACATTTAATTAAACAATTAAGGCAGCATTTTGCT ATGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288781
Insert Size	1596 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288781.1
RefSeq Size	2395 bp
RefSeq ORF	1596 bp
Locus ID	123016
UniProt ID	Q8TAM2
MW	60.4 kDa
Gene Summary	This gene encodes a protein that has been directly linked to Bardet-Biedl syndrome. The primary features of this syndrome include retinal dystrophy, obesity, polydactyly, renal abnormalities and learning disabilities. Experimentation in non-human eukaryotes suggests that this gene is expressed in ciliated cells and that it is involved in the formation of cilia. A mutation in this gene has also been implicated in nonsyndromic retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (4) includes an alternate in-frame exon in the central coding region, compared to variant 1. The encoded isoform (D) is longer, compared to isoform A.

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