PI 3 Kinase p55 gamma (PIK3R3) (NM_001303427) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LOC110117498-PIK3R3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336510 representing NM_001303427. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGCCATCTCGGCTTGTCCGTGGCCCTCGGCCCCAGGGCATGCGTTCCCCCTACCGCAGGCCAGGT ATGGGCTGGCCCAGGCCCCGATTTCCCAGGATGTTCAAGTGTAGCCGCAGAAGATATCAACAGGGTCTC CGAGGTCGAACTGCCAGCAGTGCAGCCATCAATCCTGCCACCAGGGCCATGGGTATCAACAACACCCAC ACTGACACCACCATAGTGTGGATCTTCCCACCTCAAGCTCTTCCACCAAAGCCACCTAAGCCAATGACT TCAGCAGTTCCAAATGGAATGAAGGACAGTTCTGTTTCTCTTCAGGATGCAGAATGGTACTGGGGGGAT ATTTCAAGGGAGGAGGTAAATGACAAATTGCGGGATATGCCAGATGGGACCTTCTTGGTCCGAGATGCC TCAACAAAAATGCAGGGAGATTATACTTTGACTTTGCGGAAGGGAGGCAATAATAAGTTAATAAAGATC TATCACCGGGATGGTAAATATGGCTTTTCTGATCCTCTGACATTTAATTCCGTGGTGGAGCTCATTAAC CACTATCACCATGAATCTCTTGCTCAGTACAATCCCAAACTTGATGTGAAGCTGATGTACCCAGTGTCC AGATACCAACAGGATCAGTTGGTAAAAGAAGATAATATTGATGCAGTAGGTAAAAAACTGCAAGAATAC CACTCTCAGTATCAGGAGAAGAGTAAAGAGTATGATAGGCTGTATGAAGAATATACTAGAACATCCCAG GAAATACAGATGAAGAGGACTGCAATAGAAGCTTTTAATGAAACAATTAAAATATTTGAAGAGCAGTGT CACACACAAGAACAACATAGCAAAGAATATATTGAGCGATTTCGCAGAGAGGGGAATGAAAAGGAGATT GAACGAATTATGATGAATTATGATAAATTGAAATCACGTCTGGGTGAGATTCATGATAGCAAAATGCGT CTAGAGCAGGATTTGAAGAATCAAGCTTTGGACAACCGAGAAATAGATAAAAAAATGAATAGCATCAAA CCTGACCTGATCCAGCTGCGAAAGATCCGAGATCAACACCTTGTATGGCTCAATCACAAAGGAGTGAGA CAGAAACGCCTGAATGTCTGGCTGGGAATTAAGAATGAGGATGCTGATGAGAACTATTTTATCAATGAG GAAGATGAAAACCTGCCCCATTATGATGAGAAAACCTGGTTTGTTGAGGATATCAATCGAGTACAAGCA GAGGACTTGCTTTATGGGAAACCTGATGGTGCATTCTTAATTCGTGAGAGTAGCAAGAAAGGATGCTAT GCTTGCTCTGTGGTGGCCGATGGGGAAGTGAAGCACTGTGTGATCTACAGCACTGCTCGGGGCTATGGC TTTGCAGAGCCCTACAACCTGTACAGCTCTCTGAAGGAGCTAGTGCTCCATTACCAGCAGACATCCTTG GTTCAGCACAACGACTCCCTCAACGTCAGGCTTGCCTACCCTGTTCATGCACAGATGCCCTCGCTTTGC AGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303427
Insert Size	1524 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303427.1
RefSeq Size	5098 bp
RefSeq ORF	1524 bp
Locus ID	110117499
MW	59.4 kDa
Gene Summary	This locus represents naturally occurring readthrough transcription between the neighboring genes LOC110117498 and PIK3R3. The readthrough transcript encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (3) contains an alternate 5' terminal exon and uses an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1.

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