PDE1B (NM_001288769) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HEL-S-79p; PDE1B1; PDES1B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336453 representing NM_001288769. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGAAGCAGTTGGAGAATGGGGAGATAAACATTGAGGAGCTGAAGAAAAATCTGGAGTACACAGCT TCTCTGCTGGAAGCCGTCTACATAGATGAGACACGGCAAATCTTGGACACGGAGGACGAGCTGCAGGAG CTGCGGTCAGATGCCGTGCCTTCGGAGGTGCGGGACTGGCTGGCCTCCACCTTCACCCAGCAGGCCCGG GCCAAAGGCCGCCGAGCAGAGGAGAAGCCCAAGTTCCGAAGCATTGTGCACGCTGTGCAGGCTGGGATC TTCGTGGAACGGATGTTCCGGAGAACATACACCTCTGTGGGCCCCACTTACTCTACTGCGGTTCTCAAC TGTCTCAAGAACCTGGATCTCTGGTGCTTTGATGTCTTTTCCTTGAACCAGGCAGCAGATGACCATGCC CTGAGGACCATTGTTTTTGAGTTGCTGACTCGGCATAACCTCATCAGCCGCTTCAAGATTCCCACTGTG TTTTTGATGAGTTTCCTGGATGCCTTGGAGACAGGCTATGGGAAGTACAAGAATCCTTACCACAACCAG ATCCACGCAGCCGATGTTACCCAGACAGTCCATTGCTTCTTGCTCCGCACAGGGATGGTGCACTGCCTG TCGGAGATTGAGCTCCTGGCCATCATCTTTGCTGCAGCTATCCATGATTATGAGCACACGGGCACTACC AACAGCTTCCACATCCAGACCAAGTCAGAATGTGCCATCGTGTACAATGATCGTTCAGTGCTGGAGAAT CACCACATCAGCTCTGTTTTCCGATTGATGCAGGATGATGAGATGAACATTTTCATCAACCTCACCAAG GATGAGTTTGTAGAACTCCGAGCCCTGGTCATTGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTC CAGCAAGTGAAGACCATGAAGACAGCCTTGCAACAGCTGGAGAGGATTGACAAGCCCAAGGCCCTGTCT CTACTGCTCCATGCTGCTGACATCAGCCACCCAACCAAGCAGTGGTTGGTCCACAGCCGTTGGACCAAG GCCCTCATGGAGGAATTCTTCCGTCAGGGTGACAAGGAGGCAGAGTTGGGCCTGCCCTTTTCTCCACTC TGTGACCGCACTTCCACTCTAGTGGCACAGTCTCAGATAGGGTTCATCGACTTCATTGTGGAGCCCACA TTCTCTGTGCTGACTGACGTGGCAGAGAAGAGTGTTCAGCCCCTGGCGGATGAGGACTCCAAGTCTAAA AACCAGCCCAGCTTTCAGTGGCGCCAGCCCTCTCTGGATGTGGAAGTGGGAGACCCCAACCCTGATGTG GTCAGCTTTCGTTCCACCTGGGTCAAGCGCATTCAGGAGAATAAGCAGAAATGGAAGGAACGGGCAGCA AGTGGCATCACCAACCAGATGTCCATTGACGAGCTGTCCCCCTGTGAAGAAGAGGCCCCCCCATCCCCT GCCGAAGATGAACACAACCAGAATGGGAATCTGGATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288769
Insert Size	1488 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288769.1
RefSeq Size	3083 bp
RefSeq ORF	1488 bp
Locus ID	5153
UniProt ID	Q01064
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism
MW	56.6 kDa
Gene Summary	The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] Transcript Variant: This variant (4) contains a distinct 5' UTR, lacks part of the 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1.

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