Cytochrome P450 3A5 (CYP3A5) (NM_001291830) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CP35; CYPIIIA5; P450PCN3; PCN3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336438 representing NM_001291830. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAATTACTGATGTTGGAGTTGCTGTTATTATTTATCGTATATGGGACCCGTACACATGGACTTTTT AAGAGACTGGGAATTCCAGGGCCCACACCTCTGCCTTTGTTGGGAAATGTTTTGTCCTATCGTCAGGGT CTCTGGAAATTTGACACAGAGTGCTATAAAAAGTATGGAAAAATGTGGGGAACGTATGAAGGTCAACTC CCTGTGCTGGCCATCACAGATCCCGACGTGATCAGAACAGTGCTAGTGAAAGAATGTTATTCTGTCTTC ACAAATCGAAGGTCTTTAGGCCCAGTGGGATTTATGAAAAGTGCCATCTCTTTAGCTGAGGATGAAGAA TGGAAGAGAATACGGTCATTGCTGTCTCCAACCTTCACCAGCGGAAAACTCAAGGAGATGTTCCCCATC ATTGCCCAGTATGGAGATGTATTGGTGAGAAACTTGAGGCGGGAAGCAGAGAAAGGCAAGCCTGTCACC TTGAAAGACATCTTTGGGGCCTACAGCATGGATGTGATTACTGGCACATCATTTGGAGTGAACATCGAC TCTCTCAACAATCCACAAGACCCCTTTGTGGAGAGCACTAAGAAGTTCCTAAAATTTGGTTTCTTAGAT CCATTATTTCTCTCAATAATACTCTTTCCATTCCTTACCCCAGTTTTTGAAGCATTAAATGTCTCTCTG TTTCCAAAAGATACCATAAATTTTTTAAGTAAATCTGTAAACAGAATGAAGAAAAGTCGCCTCAACGAC AAACAAAAGCACCGACTAGATTTCCTTCAGCTGATGATTGACTCCCAGAATTCGAAAGAAACTGAGTCC CACAAAGCTCTGTCTGATCTGGAGCTCGCAGCCCAGTCAATAATCTTCATTTTTGCTGGCTATGAAACC ACCAGCAGTGTTCTTTCCTTCACTTTATATGAACTGGCCACTCACCCTGATGTCCAGCAGAAACTGCAA AAGGAGATTGATGCAGTTTTGCCCAATAAGGCACCACCTACCTATGATGCCGTGGTACAGATGGAGTAC CTTGACATGGTGGTGAATGAAACACTCAGATTATTCCCAGTTGCTATTAGACTTGAGAGGACTTGCAAG AAAGATGTTGAAATCAATGGGGTATTCATTCCCAAAGGGTCAATGGTGGTGATTCCAACTTATGCTCTT CACCATGACCCAAAGTACTGGACAGAGCCTGAGGAGTTCCGCCCTGAAAGGTTCAGTAAGAAGAAGGAC AGCATAGATCCTTACATATACACACCCTTTGGAACTGGACCCAGAAACTGCATTGGCATGAGGTTTGCT CTCATGAACATGAAACTTGCTCTAATCAGAGTCCTTCAGAACTTCTCCTTCAAACCTTGTAAAGAAACA CAGATCCCCTTGAAATTAGACACGCAAGGACTTCTTCAACCAGAAAAACCCATTGTTCTAAAGGTGGAT TCAAGAGATGGAACCCTAAGTGGAGAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291830
Insert Size	1479 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291830.1
RefSeq Size	2015 bp
RefSeq ORF	1479 bp
Locus ID	1577
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Retinol metabolism
MW	56.1 kDa
Gene Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Transcript Variant: This variant (5) differs in its 5' UTR and uses an alternate start codon, compared to variant 1. The encoded isoform (4) has a shorter and distinct N-terminus, compared to isoform 1.

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