COP1 (RFWD2) (NM_001286644) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CFAP78; FAP78; RFWD2; RNF200
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336432 representing NM_001286644. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGTGGACAATATTGACCATCTGTATCCTAATTTCTTGGGAATCACATGCAGCCCAACTACAGATT CTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAACAGATCCAGAAGGAGCTA AGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGTGGCTTATACTCTCCTGTCAGTGAGGAT AGCACAGTGCCTCAATTTGAAGCTCCTTCTCCATCACACAGTAGTATTATTGATTCCACAGAATACAGC CAACCTCCAGGTTTCAGTGGCAGTTCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCA AGACGAAAACGACTTACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGT ATCTCAGATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACTCGA TATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGTTCCAGTATAGTC TCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGAGTTACAAAGAAGATTAAAGTC TATGAATATGACACTGTCATCCAGGATGCAGTGGATATTCATTACCCTGAGAATGAAATGACCTGCAAT TCGAAAATCAGCTGTATCAGTTGGAGTAGTTACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGC ACTGTTATTTTATGGGATGGATTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGT TGGAGTGTTGACTTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAG CTGTGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGTGTTAAA TTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTCCACTACTATGATCTT CGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAAGCAGTCTCTTATGCAAAGTTTGTG AGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGTCAGCTAAAACTGTGGAATGTAGGGAAACCATAC TGCCTACGTTCCTTCAAGGGTCATATCAATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTAT ATAGCTTGTGGAAGTGAAAATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACT TTTAAGTTTGATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTT AGTGCTGTGTGCTGGAGGGCACTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAACAGTCAGGGT ACAATTAAGGTGCTAGAATTGGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286644
Insert Size	1476 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286644.1
RefSeq Size	2609 bp
RefSeq ORF	1476 bp
Locus ID	64326
UniProt ID	Q8NHY2
Protein Pathways	p53 signaling pathway, Ubiquitin mediated proteolysis
MW	55.7 kDa
Gene Summary	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in its 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) has a shorter and distinct N-terminus, compared to isoform a.

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