TOM1L2 (NM_001288786) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336386 representing NM_001288786. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGTTCCTCCTGGGGAACCCGTTCAGCACACCAGTGGGGCAGTGCCTCGAAAAGGCAACAGATGGC TCCCTGCAAAGTGAGGATTGGACGTTGAATATGGAGATCTGTGACATCATCAATGAGACGGAGGAAGGG CCAAAGGATGCCATTCGAGCCCTGAAGAAGCGGCTCAACGGGAACCGGAACTACAGAGAGGTGATGCTG GCATTAACAGTGCTGGAGACATGTGTGAAGAACTGTGGCCACCGCTTCCACATCCTTGTGGCCAACCGA GATTTCATCGACAGTGTTCTGGTCAAAATTATATCTCCCAAGAACAACCCTCCCACCATTGTACAGGAC AAAGTGCTTGCTCTGATCCAGGCATGGGCTGATGCCTTTCGAAGCAGTCCTGATCTCACCGGCGTTGTG CACATATATGAGGAGCTGAAGAGGAAAGGGGTTGAATTTCCCATGGCAGACTTGGACGCTCTGTCTCCC ATACACACACCACAGCGGATTGCCAGGCTGCGGAGTGAACTGGACGTCGTTCGAGGAAACACAAAAGTC ATGTCTGAGATGTTAACAGAAATGGTCCCTGGACAGGAGGATTCATCTGATCTGGAGTTGCTGCAGGAG CTCAACAGGACCTGTCGGGCCATGCAGCAGCGCATCGTGGAGCTCATCTCCCGCGTGTCCAATGAGGAG GTCACCGAGGAGCTGCTGCATGTGAACGATGACCTCAACAACGTCTTCCTTCGATACGAGAGGTTCGAA CGATACAGGTCTGGCCGATCCGTTCAAAATGCCAGTAATGGAGTACTGAATGAAGTAACCGAAGACAAC TTAATAGACCTGGGGCCAGGGTCTCCAGCCGTGGTGAGCCCAATGGTGGGGAACACAGCGCCCCCATCT TCCCTCTCCTCCCAGCTTGCAGGCTTAGACTTGGGGACAGAGAGCGTCAGTGGCACCCTCAGTTCACTC CAGCAATGTAATCCCCGTGACGGCTTTGACATGTTTGCCCAGACGAGAGGAAACTCCTTGGCTGAGCAG CGCAAGACGGTAACCTATGAGGATCCTCAGGCTGTCGGAGGACTTGCTTCTGCACTAGACAATCGAAAA CAGAGTTCAGAAGGGACTTTTCTGTCCTCGGCCCAGAAGAGAGGTAGAGGTGGGGAGTCTGACCTGGAG CCCATAGACAGCTGGCTTATAACCCAAGGAATGATCCCCGTTGCGCAGCCATCTGTCATGGACGACATT GAGGTGTGGCTCAGGACCGACCTGAAGGGTGATGATCTGGAGGAGGGTGTCACAAGTGAAGAGTTTGAT AAATTCCTTGAAGAAAGAGCCAAAGCTGCTGAAATGGTTCCCGACCTCCCCTCGCCCCCCATGGAGGCT CCTGCCCCAGCCTCAAACCCTTCTGGCCGGAAGAAGCCAGAGCGGTCAGAGGATGCCCTCTTCGCCCTG TGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288786
Insert Size	1452 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288786.1
RefSeq Size	5753 bp
RefSeq ORF	1452 bp
Locus ID	146691
UniProt ID	Q6ZVM7
MW	53.4 kDa
Gene Summary	This gene belongs to a small gene family whose members have an N-terminal VHS domain followed by a GAT domain; domains which typically participate in vesicular trafficking. The canonical protein encoded by this gene also has a C-terminal clathrin binding motif. This protein has been shown to interact with Tollip, clathrin and ubiquitin and is thought to play a role in endosomal sorting. This gene resides in the 3.7 Mb deletion of chromosome region 17p11.2 that is associated with Smith-Magenis syndrome. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region and contains an alternate in-frame exon in the 3' coding region, compared to variant 3. It encodes isoform 4, which is shorter than isoform 3.

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