SMARCA6 (HELLS) (NM_001289074) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ICF4; LSH; Nbla10143; PASG; SMARCA6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336345 representing NM_001289074. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGTGCCGTCTAATCAGGGAGTTAAAACGATTCAATGCTGATAACAAACTTCTTTTGACTGGTACT CCCTTGCAAAACAATTTATCAGAACTTTGGTCATTGCTAAACTTTTTGTTGCCAGATGTATTTGATGAC TTGAAAAGCTTTGAGTCTTGGTTTGACATCACTAGTCTTTCTGAAACTGCTGAAGATATTATTGCTAAA GAAAGAGAACAGAATGTATTGCATATGCTGCACCAGATTTTAACACCTTTCTTATTGAGAAGACTGAAG TCTGATGTTGCTCTTGAAGTTCCTCCTAAACGAGAAGTAGTCGTTTATGCTCCACTTTCAAAGAAGCAG GAGATCTTTTATACAGCCATTGTGAACCGTACAATTGCAAACATGTTTGGATCCAGTGAGAAAGAAACA ATTGAGTTAAGTCCTACTGGTCGACCAAAACGACGAACTAGAAAATCAATAAATTACAGCAAAATAGAT GATTTCCCTAATGAATTGGAAAAACTGATCAGTCAAATACAGCCAGAGGTGGACCGAGAAAGAGCTGTT GTGGAAGTGAATATCCCTGTAGAATCTGAAGTTAATCTGAAGCTGCAGAATATAATGATGCTACTTCGT AAATGTTGTAATCATCCATATTTGATTGAATATCCTATAGACCCTGTTACACAAGAATTTAAGATCGAT GAAGAATTGGTAACAAATTCTGGGAAGTTCTTGATTTTGGATCGAATGCTGCCAGAACTAAAAAAAAGA GGTCACAAGGTGCTGCTTTTTTCACAAATGACAAGCATGTTGGACATTTTGATGGATTACTGCCATCTC AGAGATTTCAACTTCAGCAGGCTTGATGGGTCCATGTCTTACTCAGAGAGAGAAAAAAACATGCACAGC TTCAACACGGATCCAGAGGTGTTTATCTTCTTAGTGAGTACACGAGCTGGTGGCCTGGGCATTAATCTG ACTGCAGCAGATACAGTTATCATTTATGATAGTGATTGGAACCCCCAGTCGGATCTTCAGGCCCAGGAT AGATGTCATAGAATTGGTCAGACAAAGCCAGTTGTTGTTTATCGCCTTGTTACAGCAAATACTATCGAT CAGAAAATTGTGGAAAGAGCAGCTGCTAAAAGGAAACTGGAAAAGTTGATCATCCATAAAAATCATTTC AAAGGTGGTCAGTCTGGATTAAATCTGTCTAAGAATTTCTTAGATCCTAAGGAATTAATGGAATTATTA AAATCTAGAGATTATGAAAGGGAAATAAAAGGATCAAGAGAGAAGGTCATTAGTGATAAAGATCTAGAG TTGTTGTTAGATCGAAGTGATCTTATTGATCAAATGAATGCTTCAGGACCAATTAAAGAGAAGATGGGG ATATTCAAGATATTAGAAAATTCTGAAGATTCCAGTCCTGAATGTTTGTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289074
Insert Size	1434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289074.1
RefSeq Size	3082 bp
RefSeq ORF	1434 bp
Locus ID	3070
UniProt ID	Q9NRZ9
MW	55.2 kDa
Gene Summary	This gene encodes a lymphoid-specific helicase. Other helicases function in processes involving DNA strand separation, including replication, repair, recombination, and transcription. This protein is thought to be involved with cellular proliferation and may play a role in leukemogenesis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (9) uses an alternate splice site and lacks an alternate exon in the 5' coding region, and uses a downstream translation start codon, compared to variant 1. The encoded protein (isoform 9) has a shorter and distinct N-terminus compared to isoform 1.

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