STK33 (NM_001289058) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336324 representing NM_001289058. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCACAGACATCAAGCATTGGTAGTGCAGAATCTTTAATTTCACTGGAGAGAAAAAAAGAAAAAAAT ATCAACAGAGATATAACCTCCAGGAAAGATTTGCCCTCAAGAACCTCAAATGTAGAGAGAAAAGCATCT CAGCAACAATGGGGTCGGGGCAACTTTACAGAAGGAAAAGTTCCTCACATAAGGATTGAGAATGGAGCT GCTATTGAGGAAATCTATACCTTTGGAAGAATATTGGGAAAAGGGAGCTTTGGAATAGTCATTGAAGCG ACAGACAAGGAAACAGAAACGAAGTGGGCAATTAAAAAAGTGAACAAAGAAAAGGCTGGAAGCTCTGCT GTGAAGTTACTTGAACGAGAGGTGAACATTCTGAAAAGTGTAAAACATGAACACATCATACATCTGGAA CAAGTATTTGAAACGCCAAAGAAAATGTACCTTGTGATGGAGCTTTGTGAGGATGGAGAACTCAAAGAA ATTCTGGATAGGAAAGGGCATTTCTCAGAGAATGAGACAAGGTGGATCATTCAAAGTCTCGCATCAGCT ATAGCATATCTTCACAATAATGATATTGTACATAGAGATCTGAAACTGGAAAATATAATGGTTAAAAGC AGTCTTATTGATGATAACAATGAAATAAACTTAAACATAAAGGTGACTGATTTTGGCTTAGCGGTGAAG AAGCAAAGTAGGAGTGAAGCCATGCTGCAGGCCACATGTGGGACTCCTATCTATATGGCCCCTGAAGTT ATCAGTGCCCACGACTATAGCCAGCAGTGTGACATTTGGAGCATAGGCGTCGTAATGTACATGTTATTA CGTGGAGAACCACCCTTTTTGGCAAGCTCAGAAGAGAAGCTTTTTGAGTTAATAAGAAAAGGAGAACTA CATTTTGAAAATGCAGTCTGGAATTCCATAAGTGACTGTGCTAAAAGTGTTTTGAAACAACTTATGAAA GTAGATCCTGCTCACAGAATCACAGCTAAGGAACTACTAGATAACCAGTGGTTAACAGGCAATAAACTT TCTTCGGTGAGACCAACCAATGTATTAGAGATGATGAAGGAATGGAAAAATAACCCAGAAAGTGTTGAG GAAAACACAACAGAAGAGAAGAATAAGCCGTCCACTGAAGAAAAGTTGAAAAGTTACCAACCCTGGGGA AATGTCCCTGATGCCAATTACACTTCAGATGAAGAGGAGGAAAAACAGTCTACTGCTTATGAAAAGCAA TTTCCTGCAACCAGTAAGGACAACTTTGATATGTGCAGTTCAAGTTTCACATCTAGCAAACTCCTTCCA GCTGAAATCAAGGGAGAAATGGAGAAAACCCCTGTGACTCCAAGCCAAGGAACAGCAACCAAGTACCCT GCTAAATCCGGCGCCCTGTCCAGAACCAAAAAGAAACTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289058
Insert Size	1422 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289058.1
RefSeq Size	2350 bp
RefSeq ORF	1422 bp
Locus ID	65975
UniProt ID	Q9BYT3
Protein Families	Druggable Genome, Protein Kinase
MW	53.5 kDa
Gene Summary	Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (By similarity). Not essential for the survival of KRAS-dependent AML cell lines.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the coding region, and uses a downstream translation initiation codon, compared to variant 1. This results in a protein (isoform b) with a shorter C-terminus, compared to isoform a. Variants 2, 12, and 13 all encode the same isoform (b).

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