GBA3 (NM_001277225) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CBG; CBGL1; GLUC; KLRP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336311 representing NM_001277225. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCCCTTTACACTTGCTTGGTGGAGATAACTCTTCCCTTCCATTTAGAAGAAAACGTAGGAGGCTGG GATGCAGATGGAAAAGGCCCTTGTGTCTGGGACACATTTACTCATCAGGGAGGAGAGAGAGTTTTCAAG AACCAGACTGGCGATGTAGCTTGTGGCAGCTACACTCTGTGGGAGGAAGATTTGAAATGTATCAAACAG CTTGGATTGACTCATTACCGCTTCTCTCTTTCCTGGTCACGTCTGTTACCTGATGGGACGACAGGTTTC ATCAACCAGAAAGGAATTGATTATTACAACAAGATCATCGATGATTTGTTAAAAAATGGGGTTACTCCC ATTGTGACCCTCTACCACTTTGATTTGCCTCAGACTTTAGAAGACCAAGGAGGTTGGTTGTCAGAGGCA ATCATTGAATCCTTTGACAAATATGCTCAGTTTTGCTTCAGTACCTTTGGGGATCGTGTCAAGCAGTGG ATCACCATAAATGAAGCTAATGTTCTTTCTGTGATGTCATATGACTTAGGTATGTTTCCTCCGGGTATC CCTCACTTTGGGACTGGAGGTTATCAGGCAGCTCATAATTTGATTAAGGCTCATGCCAGATCCTGGCAC AGCTATGATTCCTTATTTCGAAAAAAGCAGAAAGGTATGGTGTCTCTATCACTTTTTGCGGTCTGGTTG GAACCAGCAGATCCCAACTCAGTGTCTGACCAGGAAGCTGCTAAAAGAGCCATCACTTTCCATCTGGAT TTATTTGCTAAACCCATATTCATCGATGGTGATTATCCTGAAGTTGTCAAGTCTCAGATTGCCTCCATG AGTCAAAAGCAAGGCTATCCATCATCGAGGCTTCCAGAATTCACTGAAGAAGAGAAGAAAATGATCAAA GGCACTGCTGATTTTTTTGCTGTGCAATATTATACAACTCGCTTAATCAAGTACCAGGAGAACAAGAAA GGAGAACTAGGTATTCTCCAGGATGCGGAAATTGAATTTTTTCCAGATCCATCTTGGAAAAATGTGGAT TGGATCTACGTGGTACCATGGGGAGTATGTAAACTACTGAAATATATTAAGGATACATATAATAACCCT GTAATTTACATCACTGAGAATGGGTTTCCCCAGAGTGACCCAGCGCCTCTTGATGACACTCAACGCTGG GAGTATTTCAGACAAACATTTCAGGAACTGTTCAAAGCTATCCAACTTGATAAAGTCAATCTTCAAGTA TATTGTGCATGGTCTCTTCTGGATAACTTTGAGTGGAACCAGGGATACAGCAGCCGGTTTGGTCTCTTC CACGTTGATTTTGAAGACCCAGCTAGACCCCGAGTCCCTTACACATCGGCCAAGGAATATGCCAAGATC ATCCGAAACAATGGCCTTGAAGCACATCTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001277225
Insert Size	1413 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277225.1
RefSeq Size	2091 bp
RefSeq ORF	1413 bp
Locus ID	57733
UniProt ID	Q9H227
Protein Pathways	Cyanoamino acid metabolism, Starch and sucrose metabolism
MW	54.1 kDa
Gene Summary	The protein encoded by this gene is an enzyme that can hydrolyze several types of glycosides. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals, as represented by the reference genome allele, contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Mar 2013] Transcript Variant: This variant (3, coding) contains an alternate 5' exon and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. This variant is produced from the more frequently occurring functional allele of this gene.

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