GPR123 (ADGRA1) (NM_001291085) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GPR123
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336268 representing NM_001291085. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGTGGATAGGAGTGACCGCCAGGAACATCTACAAGCAGGTGACCAAGAAGGCCCCTCTGTGCCTG GACACAGACCAGCCACCGTACCCCAGGCAGCCCCTGCTCAGGTTTTACCTCGTCAGCGGAGGGGTCCCC TTTATCATCTGTGGGGTCACGGCTGCCACGAACATCAGGAATTACGGGACAGAGGACGAGGACACGGCG TACTGCTGGATGGCCTGGGAGCCCAGCCTGGGCGCCTTCTACGGCCCAGCCGCCATCATCACCCTGGTC ACCTGTGTGTACTTCCTGGGCACCTACGTGCAGCTGCGGCGCCACCCAGGGCGCAGGTACGAGCTGCGC ACACAGCCCGAGGAGCAGCGGCGGCTGGCGACACCCGAGGGCGGCCGTGGGATCCGGCCAGGCACCCCA CCCGCACACGATGCCCCCGGCGCCTCCGTGCTGCAGAACGAGCACTCATTCCAGGCACAGCTGCGCGCC GCCGCCTTCACGCTGTTCCTGTTCACGGCCACGTGGGCCTTCGGGGCGCTGGCGGTGTCACAGGGCCAC TTCCTGGACATGGTCTTCAGCTGCCTGTACGGCGCCTTCTGCGTGACCCTGGGACTCTTCGTGCTCATC CACCACTGCGCCAAGCGTGAGGACGTGTGGCAGTGCTGGTGGGCATGCTGCCCGCCCCGCAAGGACGCC CACCCCGCACTTGACGCCAACGGGGCCGCGCTGGGCCGCGCCGCCTGCCTGCACTCGCCGGGACTGGGC CAGCCACGGGGCTTCGCGCACCCACCGGGCCCCTGCAAGATGACCAACCTGCAGGCCGCGCAGGGCCAC GCCAGTTGCCTGTCACCGGCCACCCCGTGCTGCGCCAAGATGCACTGCGAGCCACTGACGGCGGACGAG GCGCACGTGCACCTGCAGGAGGAGGGCGCCTTCGGGCACGACCCCCACCTGCACGGGTGCCTTCAGGGC AGAACTAAGCCGCCCTACTTTAGCCGGCACCCAGCAGAGGAGCCCGAGTACGCCTACCACATCCCATCC AGCCTGGATGGCAGCCCCCGCAGCTCGCGCACAGACAGCCCCCCCAGCTCTCTGGATGGCCCGGCGGGG ACACACACGCTGGCCTGCTGCACCCAGGGCGACCCCTTCCCCATGGTCACCCAGCCCGAGGGCAGTGAT GGGAGCCCTGCCCTCTACAGCTGCCCCACGCAGCCGGGCAGGGAGGCAGCGCTCGGGCCCGGCCACTTG GAGATGCTGCGGAGGACACAGTCCCTGCCCTTTGGTGGCCCCAGCCAGAACGGGCTGCCCAAGGGTAAA TTGCTAGAAGGCCTGCCGTTTGGCACCGACGGGACCGGCAACATCCGAACGGGACCCTGGAAAAACGAA ACTACTGTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291085
Insert Size	1392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291085.1
RefSeq Size	3700 bp
RefSeq ORF	1392 bp
Locus ID	84435
UniProt ID	Q86SQ6
Protein Families	Transmembrane
MW	50.1 kDa
Gene Summary	This gene encodes a protein that belongs to the adhesion family of G-protein-coupled receptors. Members of this family function in several sensory systems and regulate blood pressure, immune responses, food intake and development. A similar protein in rodents is thought to play a role in in the regulation of neuronal signaling pathways. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (2) represents the use of an alternate promoter, contains a different 5' structure and uses a downstream start codon compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.