GRB 14 (GRB14) (NM_001303422) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336208 representing NM_001303422. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTTTGAGTGCAAGAAGAGTCACTCTGCCTGCAATAACGCCAATAATTCTACAGAAAAGGGTGATT AAAGTATACAGTGAAGATGAAACCAGCAGGGCTTTAGATGTACCCAGTGACATAACGGCTCGAGATGTT TGTCAGCTGTTGATCCTGAAGAATCATTACATTGATGACCACAGCTGGACCCTTTTTGAGCACCTGCCT CACATAGGTGTAGAAAGAACAATAGAAGACCACGAACTGGTGATTGAAGTGCTATCCAACTGGGGGATA GAAGAAGAAAACAAACTATACTTTAGAAAAAATTATGCCAAATATGAGTTCTTTAAAAACCCAATGTAT TTTTTTCCAGAGCATATGGTATCTTTTGCAACTGAAACCAATGGTGAAATATCCCCCACACAGATTTTG CAGATGTTTCTGAGTTCAAGCACATATCCTGAAATTCATGGTTTCTTACATGCGAAAGAACAGGGAAAG AAGTCTTGGAAAAAAATTTACTTTTTTCTAAGAAGATCTGGTTTATATTTTTCTACTAAAGGAACATCA AAGGAACCGCGGCATTTGCAGTTTTTCAGCGAATTTGGCAATAGTGATATTTATGTGTCACTGGCAGGC AAAAAAAAACATGGAGCACCGACTAACTATGGATTCTGCTTTAAGCCTAACAAAGCGGGAGGGCCCCGA GACCTGAAAATGCTCTGTGCAGAAGAAGAGCAGAGTAGGACGTGCTGGGTGACCGCGATTAGATTGCTT AAGTATGGCATGCAGCTGTACCAGAATTATATGCATCCATATCAAGGTAGAAGTGGCTGCAGTTCACAG AGCATATCACCTATGAGAAGTATATCAGAGAATTCCCTGGTAGCAATGGACTTCTCAGGCCAGAAAAGC AGAGTTATAGAAAATCCCACTGAAGCCCTTTCAGTTGCGGTTGAAGAAGGACTCGCTTGGAGGAAAAAA GGATGTTTACGCCTGGGCACTCACGGTAGCCCCACTGCCTCTTCACAGAGCTCTGCCACAAACATGGCT ATCCACCGGTCCCAGCCATGGTTTCACCACAAAATTTCTAGAGATGAGGCTCAGCGATTGATTATTCAG CAAGGACTTGTGGATGGAGTTTTCTTGGTACGGGATAGTCAGAGTAACCCCAAAACTTTCGTACTGTCA ATGAGTCATGGACAAAAAATAAAGCACTTTCAAATTATACCAGTAGAAGATGACGGTGAAATGTTCCAC ACACTGGATGATGGCCACACAAGATTTACAGATCTAATACAGCTGGTGGAGTTCTATCAACTCAATAAG GGCGTTCTTCCTTGCAAGTTGAAACATTATTGTGCTAGGATTGCTCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303422
Insert Size	1362 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303422.1
RefSeq Size	2165 bp
RefSeq ORF	1362 bp
Locus ID	2888
UniProt ID	Q14449
MW	51.9 kDa
Gene Summary	The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. This protein likely has an inhibitory effect on receptor tyrosine kinase signaling and, in particular, on insulin receptor signaling. This gene may play a role in signaling pathways that regulate growth and metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.