ENTPD3 (NM_001291961) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CD39L3; HB6; NTPDase-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336200 representing NM_001291961. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCACTGTGCTGACCCGCCAACCATGTGAGCAAGCAGGCCTCAAGGCCCTCTACCGAACTCCAACC ATCATTGCCTTGGTGGTCTTGCTTGTGAGTATTGTGGTACTTGTGAGTATCACTGTCATCCAGATCCAC AAGCAAGAGGTCCTCCCTCCAGGACTGAAGTATGGTATTGTGCTGGATGCCGGGTCTTCAAGAACCACA GTCTACGTGTATCAATGGCCAGCAGAAAAAGAGAATAATACCGGAGTGGTCAGTCAAACCTTCAAATGT AGTGTGAAAGGCTCTGGAATCTCCAGCTATGGAAATAACCCCCAAGATGTCCCCAGAGCCTTTGAGGAG TGTATGCAAAAAGTCAAGGGGCAGGTTCCATCCCACCTCCACGGATCCACCCCCATTCACCTGGGAGCC ACGGCTGGGATGCGCTTGCTGAGGTTGCAAAATGAAACAGCAGCTAATGAAGTCCTTGAAAGCATCCAA AGCTACTTCAAGTCCCAGCCCTTTGACTTTAGGGGTGCTCAAATCATTTCTGGGCAAGAAGAAGGGGTA TATGGATGGATTACAGCCAACTATTTAATGGGAAATTTCCTGGAGAAGAACCTGTGGCACATGTGGGTG CACCCGCATGGAGTGGAAACCACGGGTGCCCTGGACTTAGGTGGTGCCTCCACCCAAATATCCTTCGTG GCAGGAGAGAAGATGGATCTGAACACCAGCGACATCATGCAGGTGTCCCTGTATGGCTACGTATACACG CTCTACACACACAGCTTCCAGTGCTATGGCCGGAATGAGGCTGAGAAGAAGTTTCTGGCAATGCTCCTG CAGAATTCTCCTACCAAAAACCATCTCACCAATCCCTGTTACCCTCGGGATTATAGCATCAGCTTCACC ATGGGCCATGTATTTGATAGCCTGTGCACTGTGGACCAGAGGCCAGAAAGTTATAACCCCAATGATGTC ATCACTTTTGAAGGAACTGGGGACCCATCTCTGTGTAAGGAGAAGGTGGCTTCCATATTTGACTTCAAA GCTTGCCATGATCAAGAAACCTGTTCTTTTGATGGGGTTTATCAGCCAAAGATTAAAGGGCCATTTGTG GCTTTTGCAGGATTCTACTACACAGCCAGTGCTTTAAATCTTTCAGGTAGCTTTTCCCTGGACACCTTC AACTCCAGCACCTGGAATTTCTGCTCACAGAATTGGAGTCAGCTCCCACTGCTGCTCCCCAAATTTGAT GAGGTATATGCCCGCTCTTACTGCTTCTCAGCCAACTACATCTACCACTTGTTTGTGAACGGTTACAAA TTCACAGAGGAGACTTGGCCCCAAATACACTTTGAAAAAGAAGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291961
Insert Size	1359 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291961.1
RefSeq Size	1730 bp
RefSeq ORF	1359 bp
Locus ID	956
UniProt ID	O75355
Protein Families	Transmembrane
Protein Pathways	Purine metabolism, Pyrimidine metabolism
MW	50.8 kDa
Gene Summary	This gene encodes a plasma membrane-bound divalent cation-dependent E-type nucleotidase. The encoded protein is involved in the regulation of extracellular levels of ATP by hydrolysis of it and other nucleotides. Multiple transcript variants have been described. [provided by RefSeq, May 2014] Transcript Variant: This variant (3) differs in the 5' UTR and includes a different terminal 3' exon compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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