Pescadillo (PES1) (NM_001282328) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NOP7; PES
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336195 representing NM_001282328. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGCTTCCTGTTTTCCACCTTCCCGCGGACTGGCAAGTGCCACGTGCAGACCATTCAGCTGTGCCGC CGGCTCACTGTGGAGTTCATGCACTACATTATCGCTGCCCGTGCCCTGCGCAAGGTCTTCCTGTCCATC AAAGGCATTTACTACCAGGCCGAGGTACTGGGGCAGCCCATCGTGTGGATCACTCCCTATGCCTTCTCC CATGACCACCCGACAGACGTGGACTACAGGGTCATGGCCACCTTCACCGAGTTCTACACCACGCTGCTG GGCTTTGTCAACTTCCGCCTTTACCAGTTGCTCAACCTCCACTATCCCCCGAAGCTCGAGGGTCAGGCC CAAGCAGAGGCAAAGGCCGGTGAGGGCACCTACGCGTTGGACTCCGAGAGTTGTATGGAGAAACTGGCA GCCCTCAGTGCCAGCCTGGCCCGCGTGGTGGTGCCTGCCACAGAGGAGGAGGCCGAGGTGGATGAGTTT CCCACCGATGGGGAGATGTCAGCGCAGGAGGAAGACCGCAGGAAGGAGCTGGAGGCGCAGGAGAAGCAC AAGAAGCTTTTTGAGGGCCTGAAGTTCTTCCTGAACCGAGAGGTGCCCCGTGAGGCCCTGGCCTTCATC ATCAGGAGTTTTGGTGGGGAAGTGTCCTGGGACAAATCTTTGTGCATTGGGGCCACCTATGACGTCACA GACTCCCGCATCACCCATCAGATTGTCGACCGGCCTGGGCAGCAGACCTCAGTCATTGGCAGGTGCTAC GTGCAGCCCCAGTGGGTGTTTGACTCAGTGAACGCCAGGCTCCTTCTCCCCGTGGCAGAGTACTTCTCT GGGGTGCAGCTGCCCCCACACCTTTCACCCTTTGTGACCGAGAAGGAAGGAGATTACGTTCCACCTGAG AAGCTGAAGCTGCTGGCTCTGCAGCGGGGAGAGGACCCAGGAAACCTGAATGAGTCAGAAGAGGAGGAG GAAGAGGACGACAACAACGAAGGTGATGGTGATGAAGAGGGAGAAAATGAGGAGGAGGAGGAAGATGCA GAGGCTGGTTCAGAAAAGGAGGAAGAGGCCCGGCTGGCAGCCCTGGAAGAGCAGAGGATGGAGGGGAAG AAGCCCAGGGTGATGGCAGGCACCTTGAAGCTGGAGGATAAGCAGCGGCTGGCCCAGGAGGAGGAGAGT GAGGCCAAGCGCCTGGCCATTATGATGATGAAGAAGCGGGAGAAGTACCTGTACCAGAAGATCATGTTT GGCAAGAGGCGAAAAATCCGAGAGGCCAACAAGCTGGCGGAGAAGCGGAAAGCCCACGATGAGGCGGTG AGGTCTGAGAAGAAGGCCAAGAAGGCAAGGCCGGAGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282328
Insert Size	1350 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282328.1
RefSeq Size	2757 bp
RefSeq ORF	1350 bp
Locus ID	23481
MW	51.5 kDa
Gene Summary	This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9. [provided by RefSeq, Aug 2011] Transcript Variant: This variant (4) represents use of an alternate promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG, and the resulting isoform (3) has a shorter N-terminus compared to isoform 1. Variants 2 and 4 encode the same protein (isoform 3).

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