CYP39A1 (NM_001278738) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336193 representing NM_001278738. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAACTAATTTCCCCAACAGTGATTATAATCCTGGGTTGCCTTGCTCTGTTCTTACTCCTTCAGCGG AAGAATTTGCGTAGACCCCCGTGCATCAAGGGCTGGATTCCTTGGATTGGAGTTGGATTTGAGTTTGGG AAAGCCCCTCTAGAATTTATAGAGAAAGCAAGAATCAAGTATGGACCAATATTTACAGTCTTTGCTATG GGAAACCGAATGACCTTTGTTACTGAAGAAGAAGGAATTAATGTGTTTCTAAAATCCAAAAAAGTAGAT TTTGAACTAGCAGTGCAAAATATCGTTTATCGTACAGGGAAAATGGGGACTGTCAATCTCCATCAGTTT ACTGGGCAACTGACTGAAGAATTACATGAACAACTGGAGAATTTAGGCACTCATGGGACAATGGACCTG AACAACTTAGTAAGACATCTCCTTTATCCAGTCACAGTGAATATGCTCTTTAATAAAAGTTTGTTTTCC ACAAACAAGAAAAAAATCAAGGAGTTCCATCAGTATTTTCAAGTTTATGATGAAGATTTTGAGTATGGG TCCCAGTTGCCAGAGTGTCTTCTAAGAAACTGGTCAAAATCCAAAAAGTGGTTCCTGGAACTGTTTGAG AAAAACATTCCAGATATAAAAGCATGTAAATCTGCAAAAGATAATTCCATGACATTATTGCAAGCTACG CTGGATATTGTAGAGACGGAAACAAGTAAGGAAAACTCACCCAATTATGGGCTCTTACTGCTTTGGGCT TCTCTGTCTAATGCTGTTCCTGTTGCATTTTGGACACTTGCATACGTCCTTTCTCATCCTGATATCCAC AAGGCCATTATGGAAGGCATATCTTCTGTGTTTGGCAAAGCAGGCAAAGATAAGATTAAAGTGTCTGAG GATGACCTGGAGAATCTCCTTCTAATTAAATGGTGTGTTTTGGAAACCATTCGTTTAAAAGCTCCTGGT GTCATTACTAGAAAAGTGGTGAAGCCTGTGGAAATTTTGAATTACATCATTCCTTCTGGTGACTTGTTG ATGTTGTCTCCATTTTGGCTGCATAGAAATCCAAAGTATTTTCCTGAGCCTGAATTGTTCAAACCTGAA CGTTGGAAAAAGGCAAATTTAGAGAAGCACTCTTTCTTGGACTGCTTCATGGCATTTGGAAGCGGGAAG TTCCAGTGTCCTGCAAGGTGGTTTGCTCTGTTAGAGGTTCAGATGTGTATTATTTTAATACTTTATAAA TATGACTGTAGTCTTCTGGACCCATTACCCAAACAGAGTTATCTCCATTTGGTGGGTGTCCCCCAGCCG GAAGGGCAATGCCGAATTGAATATAAACAAAGAATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278738
Insert Size	1350 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278738.1
RefSeq Size	2384 bp
RefSeq ORF	1350 bp
Locus ID	51302
UniProt ID	Q9NYL5
Protein Families	Druggable Genome, P450, Transmembrane
Protein Pathways	Primary bile acid biosynthesis
MW	51.8 kDa
Gene Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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