CTBP2 (NM_001290215) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC336165 representing NM_001290215. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCTTGTGGATAAGCACAAAGTCAAGAGACAGCGATTGGACAGAATTTGTGAAGGTATCCGCCCC CAGATCATGAACGGCCCCCTGCACCCCCGCCCCCTGGTGGCGCTGCTGGACGGCCGCGACTGCACTGTG GAGATGCCCATCCTGAAGGACCTGGCCACTGTGGCCTTCTGTGACGCGCAGTCGACGCAGGAAATCCAC GAGAAGGTTCTAAACGAAGCCGTGGGCGCCATGATGTACCACACCATCACCCTCACCAGGGAGGACCTG GAGAAGTTCAAGGCCCTGAGAGTGATCGTGCGGATAGGCAGTGGCTATGACAACGTGGACATCAAGGCT GCCGGCGAGCTCGGAATTGCCGTGTGCAACATCCCGTCTGCAGCCGTGGAAGAGACAGCGGACTCTACC ATCTGCCACATCCTCAACCTGTACCGGAGGAACACGTGGCTGTACCAGGCACTGCGGGAAGGCACGCGG GTTCAGAGCGTGGAGCAGATCCGCGAGGTGGCCTCGGGAGCGGCCCGCATCCGTGGGGAGACGCTGGGC CTCATTGGCTTTGGTCGCACGGGGCAGGCGGTTGCAGTTCGAGCCAAGGCCTTTGGATTCAGCGTCATA TTTTATGACCCCTACTTGCAGGATGGGATCGAGCGGTCCCTGGGCGTGCAGAGGGTCTACACCCTGCAG GATTTGCTGTATCAGAGCGACTGCGTCTCCTTGCACTGCAATCTCAACGAACATAACCACCACCTCATC AATGACTTTACCATAAAGCAGATGAGGCAGGGAGCATTCCTTGTGAACGCAGCCCGTGGCGGCCTGGTG GACGAGAAAGCCTTAGCACAAGCCCTCAAGGAGGGCAGGATACGAGGGGCAGCCCTCGACGTGCATGAG TCAGAGCCCTTCAGCTTTGCTCAGGGTCCGTTGAAAGATGCCCCGAATCTCATCTGCACTCCTCACACT GCCTGGTACAGTGAGCAGGCGTCACTGGAGATGAGGGAGGCAGCTGCCACCGAGATCCGCCGAGCCATC ACAGGTCGCATCCCAGAAAGCTTAAGAAATTGTGTGAACAAGGAATTCTTTGTCACATCAGCGCCTTGG TCAGTAATAGACCAGCAAGCAATTCATCCTGAGCTCAATGGTGCCACATACAGATATCCGCCAGGCATC GTGGGTGTGGCTCCAGGAGGACTTCCTGCAGCCATGGAAGGGATCATCCCTGGAGGCATCCCAGTGACT CACAACCTCCCGACAGTGGCACATCCTTCCCAAGCGCCCTCTCCCAACCAGCCCACAAAACACGGGGAC AATCGAGAGCACCCCAACGAGCAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001290215
Insert Size	1338 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290215.1
RefSeq Size	3393 bp
RefSeq ORF	1338 bp
Locus ID	1488
UniProt ID	P56545
Protein Families	Stem cell - Pluripotency, Stem cell relevant signaling - Wnt Signaling pathway
Protein Pathways	Chronic myeloid leukemia, Notch signaling pathway, Pathways in cancer, Wnt signaling pathway
MW	48.9 kDa
Gene Summary	This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21.3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (5) contains a distinct 5' UTR and 5' CDS, compared to variant 2. Variants 1, 3, 4, and 5-8 all encode the same isoform (1), which has a distinct N-terminus that is 540 aa shorter than the N-terminus of isoform 2. The protein is thought to bind to the C-terminus of the adenovirus E1A proteins. Studies in mice suggest that this protein is involved in transcriptional repression. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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