NUDT12 (NM_001300741) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336162 representing NM_001300741. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTTCTGTAAAAAGAAGTCTGAAGCAAGAAATAGTTACTCAGTTTCACTGTTCAGCTGCTGAAGGA GATATTGCCAAGTTAACAGGAATACTCAGTCATTCTCCATCTCTTCTCAATGAAACTTCTGAAAATGGC TGGACTGCTTTAATGTGTGACAGATCAATTGTCAATAAATCAAGGCAGACTGCACTGGACATTGCTGTA TTTTGGGGTTATAAGCATATAGCTAATTTACTAGCTACTGCTAAAGGTGGGAAGAAGCCTTGGTTCCTA ACGAATGAAGTGGAAGAATGTGAAAATTATTTTAGCAAAACACTACTGGACCGGAAAAGTGAAAAGAGA AATAATTCTGACTGGCTGCTAGCTAAAGAAAGCCATCCAGCCACAGTTTTTATTCTTTTCTCAGATTTA AATCCCTTGGTTACTCTAGGTGGCAATAAAGAAAGTTTCCAACAGCCAGAAGTTAGGCTTTGTCAGCTG AACTACACAGATATAAAGGATTATTTGGCCCAGCCTGAGAAGATCACCTTGATTTTTCTTGGAGTAGAA CTTGAAATAAAAGACAAACTACTTAATTATGCTGGTGAAGTCCCGAGAGAGGAGGAAGATGGATTGGTT GCCTGGTTTGCTCTAGGTATAGATCCTATTGCTGCTGAAGAATTCAAGCAAAGACATGAAAATTGTTAC TTTCTTCATCCTCCTATGCCAGCCCTTCTGCAATTGAAAGAAAAAGAAGCTGGGGTTGTAGCTCAAGCA AGATCTGTTCTTGCCTGGCACAGTCGATACAAGTTTTGCCCAACCTGTGGAAATGCAACTAAAATTGAA GAAGGTGGCTATAAGAGATTATGTTTAAAAGAAGACTGTCCTAGTCTCAATGGCGTCCATAATACCTCA TACCCAAGAGTTGATCCAGTAGTAATCATGCAAGTTATTCATCCAGATGGGACCAAATGCCTTTTAGGC AGGCAGAAAAGATTTCCCCCAGGCATGTTTACTTGCCTTGCTGGATTTATTGAGCCTGGAGAGACAATA GAAGATGCTGTTAGGAGAGAAGTAGAAGAGGAAAGTGGAGTCAAAGTTGGCCATGTTCAGTATGTTGCT TGTCAACCATGGCCAATGCCTTCCTCCTTAATGATTGGTTGCTTAGCTCTAGCAGTGTCTACAGAAATT AAAGTTGACAAGAATGAAATAGAGGATGCCCGCTGGTTCACTAGAGAACAGGTCCTGGATGTTCTGACC AAAGGGAAGCAGCAGGCATTCTTTGTGCCACCAAGCCGAGCTATTGCACATCAATTAATCAAACACTGG ATTAGAATAAATCCTAATCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300741
Insert Size	1335 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300741.1
RefSeq Size	3460 bp
RefSeq ORF	1335 bp
Locus ID	83594
UniProt ID	Q9BQG2
Protein Pathways	Nicotinate and nicotinamide metabolism
MW	50.1 kDa
Gene Summary	Nucleotides are involved in numerous biochemical reactions and pathways within the cell as substrates, cofactors, and effectors. Nudix hydrolases, such as NUDT12, regulate the concentrations of individual nucleotides and of nucleotide ratios in response to changing circumstances (Abdelraheim et al., 2003 [PubMed 12790796]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) has an alternate splcie site in the 5' coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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