SKAR (POLDIP3) (NM_001278657) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PDIP3; PDIP46; SKAR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336130 representing NM_001278657. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGACATCTCCCTGGACGAACTCATCAGGAAGCGCGGGGCGGCGGCGAAAGGACGGCTTAATGCC AGACCGGGAGTTGGAGGTGTCCGATCTCGAGTTGGGATCCAGCAAGGCCTTCTCAGCCAGTCAACACGC ACAGCCACCTTCCAGCAGAGATTTGATGCCCGGCAGAAGATTGGCCTCTCAGATGCCCGGCTCAAACTG GGAGTCAAGGATGCCCGGGAGAAGCTTTTGCAGAAAGATGCCCGATTTCGAATCAAAGGGAAAGTGCAG GATGCCAGAGAGATGTTGAACTCTCGCAAGCAGCAGACCACGGTGCCCCAGAAGCCCCGCCAGGTTGCT GATGCCCGGGAGAAGATCAGCTTGAAGAGGAGTTCCCCTGCTGCCTTCATAAACCCACCCATTGGGACA GTGACCCCTGCTCTGAAGCTCACCAAAACCATCCAGAGAATGCATGTGGACCCTGTGGGGTGGATTCTG ACCACTGAGTGTTTCCAGGTTCCACAGCAGAAAGCCATGGCACCACTTCATCCCCATCCTGCCGGAATG AGAATCAATGTTGTCAATAACCACCAGGCCAAACAGAATTTATATGACCTGGATGAAGATGATGATGGT ATAGCTTCCGTTCCTACTAAACAGATGAAGTTTGCAGCCTCAGGCGGCTTTCTCCACCACATGGCTGGG CTAAGCAGTTCCAAGCTTTCCATGTCCAAGGCCCTCCCTCTCACCAAAGTGGTTCAGAATGATGCATAC ACAGCTCCTGCTCTCCCTTCCTCTATTCGAACAAAAGCCTTGACCAACATGTCCCGGACACTGGTGAAC AAGGAAGAACCCCCCAAAGAGCTGCCAGCTGCTGAGCCTGTTCTCAGCCCATTGGAAGGCACCAAGATG ACTGTGAATAATCTGCACCCTCGAGTCACTGAGGAGGACATTGTTGAGCTTTTCTGTGTGTGTGGGGCC CTCAAGCGAGCTCGACTGGTCCATCCTGGGGTAGCGGAGGTGGTGTTTGTGAAAAAGGACGATGCCATC ACCGCATATAAGAAGTACAACAACCGGTGTCTGGACGGGCAGCCGATGAAGTGCAACCTTCACATGAAT GGGAATGTTATCACCTCAGACCAGCCCATCCTGCTGCGGCTGAGTGACAGCCCATCAATGAAAAAGGAG AGCGAGCTGCCTCGCAGGGTGAACTCTGCCTCCTCCTCCAACCCCCCTGCCGAAGTGGACCCTGACACC ATCCTGAAGGCACTCTTCAAGTCCTCAGGGGCCTCTGTGACCACGCAGCCCACAGAATTCAAAATCAAG CTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278657
Insert Size	1317 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278657.1
RefSeq Size	3511 bp
RefSeq ORF	1317 bp
Locus ID	84271
MW	48.1 kDa
Gene Summary	This gene encodes an RRM (RNA recognition motif)-containing protein that participates in the regulation of translation by recruiting ribosomal protein S6 kinase beta-1 to mRNAs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (3) is longer than isoform 1.

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