P2Y6 (P2RY6) (NM_001277208) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	P2Y6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336070 representing NM_001277208. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGTCAGCATTGGTGTAAAGGACTGGAGCTTCAGGGTTCTCGGGATTCGAGTCCTGGCCCTGCTGCT TGCCAGCTCTGTGCTGTGGCAAGTTACTTCCTCTCCCCAGGTCTCTCGGTTTCCTCATCTGCTGCCTCT CCAGACTTCTGCCAGAACATTGCACGCGACAGTTTCAGGCACAGAACTGACTGGCAGCAGGGGCTGCTC CACGAGTGGGAATTTGCTCCAGCACTTCACGGACTGCAAGCGAGGCACTTGCTAACTCTTGGCCTCCCT GAACATAGGAAACCCACCTGGGCAGCCATGGAATGGGACAATGGCACAGGCCAGGCTCTGGGCTTGCCA CCCACCACCTGTGTCTACCGCGAGAACTTCAAGCAACTGCTGCTGCCACCTGTGTATTCGGCGGTGCTG GCGGCTGGCCTGCCGCTGAACATCTGTGTCATTACCCAGATCTGCACGTCCCGCCGGGCCCTGACCCGC ACGGCCGTGTACACCCTAAACCTTGCTCTGGCTGACCTGCTATATGCCTGCTCCCTGCCCCTGCTCATC TACAACTATGCCCAAGGTGATCACTGGCCCTTTGGCGACTTCGCCTGCCGCCTGGTCCGCTTCCTCTTC TATGCCAACCTGCACGGCAGCATCCTCTTCCTCACCTGCATCAGCTTCCAGCGCTACCTGGGCATCTGC CACCCGCTGGCCCCCTGGCACAAACGTGGGGGCCGCCGGGCTGCCTGGCTAGTGTGTGTAGCCGTGTGG CTGGCCGTGACAACCCAGTGCCTGCCCACAGCCATCTTCGCTGCCACAGGCATCCAGCGTAACCGCACT GTCTGCTATGACCTCAGCCCGCCTGCCCTGGCCACCCACTATATGCCCTATGGCATGGCTCTCACTGTC ATCGGCTTCCTGCTGCCCTTTGCTGCCCTGCTGGCCTGCTACTGTCTCCTGGCCTGCCGCCTGTGCCGC CAGGATGGCCCGGCAGAGCCTGTGGCCCAGGAGCGGCGTGGCAAGGCGGCCCGCATGGCCGTGGTGGTG GCTGCTGCCTTTGCCATCAGCTTCCTGCCTTTTCACATCACCAAGACAGCCTACCTGGCAGTGCGCTCG ACGCCGGGCGTCCCCTGCACTGTATTGGAGGCCTTTGCAGCGGCCTACAAAGGCACGCGGCCGTTTGCC AGTGCCAACAGCGTGCTGGACCCCATCCTCTTCTACTTCACCCAGAAGAAGTTCCGCCGGCGACCACAT GAGCTCCTACAGAAACTCACAGCCAAATGGCAGAGGCAGGGTCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001277208
Insert Size	1290 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277208.1
RefSeq Size	2466 bp
RefSeq ORF	1290 bp
Locus ID	5031
UniProt ID	Q15077
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
MW	47.5 kDa
Gene Summary	The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Mar 2013] Transcript Variant: This variant (9) contains an alternate 5'-most exon and initiates translation at an alternate start codon so that the 5' UTR and 5' coding region differ compared to variant 1. The encoded isoform (2) has a longer N-terminus than isoform 1.

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