Transmembrane Protein 175 (TMEM175) (NM_001297424) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hTMEM175
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336026 representing NM_001297424. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCTTTCTCATCGTGACAGTGGCCTGGGCAGCACACACAAGGTTGTTCCAAGTTGTTGGGAAAACA GACGACACACTTGCCCTGCTCAACCTGGCCTGCATGATGACCATCACCTTCCTGCCTTACACGTTTTCG TTAATGGTGACCTTCCCTGATGTGCCTCTGGGCATCTTCTTGTTCTGTGTGTGTGTGATCGCCATTGGG GTCGTGCAGGCACTGATTGTGGGGTACGCATTCCACTTCCCGCACCTGCTGAGCCCGCAGATCCAGCGC TCTGCCCACAGGGCTCTGTACCGACGACACGTCCTGGGCATCGTCCTCCAAGGCCCGGCCCTGTGCTTT GCAGCGGCCATCTTCTCTCTCTTCTTTGTCCCCTTGTCTTACCTGCTGATGGTGACTGTCATCCTCCTC CCCTATGTCAGCAAGGTCACCGGCTGGTGCAGAGACAGGCTCCTGGGCCACAGGGAGCCCTCGGCTCAC CCAGTGGAAGTCTTCTCGTTTGACCTCCACGAGCCACTCAGCAAGGAGCGCGTGGAAGCCTTCAGCGAC GGAGTCTACGCCATCGTGGCCACGCTTCTCATCCTGGACATCTGCGAAGACAACGTCCCGGACCCCAAG GATGTGAAGGAGAGGTTCAGCGGCAGCCTCGTGGCCGCCCTGAGTGCGACCGGGCCGCGCTTCCTGGCG TACTTCGGCTCCTTCGCCACAGTGGGACTGCTGTGGTTCGCCCACCACTCACTCTTCCTGCATGTGCGC AAGGCCACGCGGGCCATGGGGCTGCTGAACACGCTCTCGCTGGCCTTCGTGGGTGGCCTCCCACTAGCC TACCAGCAGACCTCGGCCTTCGCCCGGCAGCCCCGCGATGAGCTGGAGCGCGTGCGTGTCAGCTGCACC ATCATCTTCCTGGCCAGCATCTTCCAGCTGGCCATGTGGACCACGGCGCTGCTGCACCAGGCGGAGACG CTGCAGCCCTCGGTGTGGTTTGGCGGCCGGGAGCATGTGCTCATGTTCGCCAAGCTGGCGCTGTACCCC TGTGCCAGCCTGCTGGCCTTCGCCTCCACCTGCCTGCTGAGCAGGTTCAGTGTGGGCATCTTCCACCTC ATGCAGATCGCCGTGCCCTGCGCCTTCCTGTTGCTGCGCCTGCTCGTGGGCCTGGCCCTGGCCACCCTG CGGGTCCTGCGGGGCCTCGCCCGGCCCGAACACCCCCCGCCAGCCCCCACGGGCCAGGACGACCCACAG TCCCAGCTCCTCCCTGCCCCCTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297424
Insert Size	1269 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297424.1
RefSeq Size	1661 bp
RefSeq ORF	1269 bp
Locus ID	84286
Protein Families	Transmembrane
MW	46.6 kDa
Gene Summary	Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in its 5' UTR and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2, 3, and 4 encode the same isoform.

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