RBMY1F (NM_001303410) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	YRRM2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336009 representing NM_001303410. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTAGAAGCAGATCATCCTGGCAAGCTTTTCATTGGTGGCCTCAATAGAGAAACCAATGAGAAGATG CTTAAAGCAGTATTTGGGAAACATGGTCCCATATCAGAAGTTCTTTTGATAAAGGATCGAACCAGCAAA TCCAGAGGCTTTGCATTTATTACTTTTGAGAACCCTGCAGATGCTAAGAATGCTGCGAAAGATATGAAT GGAACGTCTTTGCATGGAAAAGCAATAAAAGTAGAACAAGCCAAGAAACCATCTTTTCAAAGTGGTGGT AGGCGGAGACCACCAGCTTCTTCGAGAAACAGAAGCCCTTCAGGAAGTCTGAGATCTGCAAGAGGAAGC AGTGGAGGAACAAGAGGGTGGCTTCCCTCACATGAAGGGCACCTGGATGATGGTGGATACACTCCTGAT CTCAAGATGAGTTATTCTAGGGGACTCATTCCAGTTAAAAGAGGTCCATCTTCAAGAAGTGGAGGTCCT CCTCCGAAAAAATCTGCTCCTTCTGCTGTGGCAAGAAGCAATAGTTGGATGGGAAGCCAAGGTCCCATG TCACAAAGAAGAGAGAATTATGGAGTTCCTCCACGCAGAGCGACAATATCTTCCTGGAGAAATGATCGC ATGTCAACAAGACATGATGGTTATGCAACTAACGATGGAAATCATCCAAGTTGCCAAGAAACGAGGGAT TATGCTCCACCATCTAGAGGCTATGCATACCGTGATAATGGTCATTCTAATCGGGATGAACATTCCTCT AGAGGATATAGAAATCATCGAAGTTCCCGAGAAACTAGGGATTATGCTCCACCATCTAGAGGCCATGCA TACCGTGATTATGGTCATTCTCGTCGGGATGAAAGTTATTCTAGAGGATACAGAAATCATCGAAGTTCC CGAGAAACTAGGGAGTATGCTCCACCATCTAGAGGCCATGGATACCGTGATTATGGTCATTCTCGTCGA CATGAAAGTTATTCTAGAGGATATAGAAATCATCCAAGTTCCCGAGAAACCAGGGATTATGCTCCACCA CATAGAGACTATGCATACCGTGATTATGGTCATTCTAGTTGGGATGAACATTCCTCTAGAGGATATAGT TATCATGATGGCTACGGTGAGGCCCTTGGTAGAGATCATTCTGAACATCTAAGTGGAAGTTCTTATAGA GATGCACTTCAGAGGGACCTCTCATGGTGCACCACCTGCAAGAGGGCCTCGGATGTCTTATGGTGGAAG CACCTGCCACGCATATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303410
Insert Size	1260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001303410.1
RefSeq Size	1890 bp
RefSeq ORF	1260 bp
Locus ID	159163
UniProt ID	Q15415
MW	47.5 kDa
Gene Summary	This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes, although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3, along with two copies of PTPN13-like, Y-linked. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, which results in a frameshift compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

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