SP7 (NM_001300837) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	OI11; OI12; osterix; OSX
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335966 representing NM_001300837. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTGACGGCAGCGTGCAGCAAATTTGGTGGCTCTAGCCCTCTGCGGGACTCAACAACTCTGGGCAAA GCAGGCACAAAGAAGCCGTACTCTGTGGGCAGTGACCTTTCAGCCTCCAAAACCATGGGGGATGCTTAT CCAGCCCCCTTTACAAGCACTAATGGGCTCCTTTCACCTGCAGGCAGTCCTCCAGCACCCACCTCAGGC TATGCTAATGATTACCCTCCCTTTTCCCACTCATTCCCTGGGCCCACAGGCACCCAGGACCCTGGGCTA CTAGTGCCCAAGGGGCACAGCTCTTCTGACTGTCTGCCCAGTGTCTACACCTCTCTGGACATGACACAC CCCTATGGCTCCTGGTACAAGGCAGGCATCCATGCAGGCATTTCACCAGGCCCAGGCAACACTCCTACT CCATGGTGGGATATGCACCCTGGAGGCAACTGGCTAGGTGGTGGGCAGGGCCAGGGTGATGGGCTGCAA GGGACACTGCCCACAGGTCCAGCTCAGCCTCCACTGAACCCCCAGCTGCCCACCTACCCATCTGACTTT GCTCCCCTTAATCCAGCCCCCTACCCAGCTCCCCACCTCTTGCAACCAGGGCCCCAGCATGTCTTGCCC CAAGATGTCTATAAACCCAAGGCAGTGGGAAATAGTGGGCAGCTAGAAGGGAGTGGTGGAGCCAAACCC CCACGGGGTGCAAGCACTGGGGGTAGTGGTGGATATGGGGGCAGTGGGGCAGGGCGCTCCTCCTGCGAC TGCCCTAATTGCCAGGAGCTAGAGCGGCTGGGAGCAGCAGCGGCTGGGCTGCGGAAGAAGCCCATCCAC AGCTGCCACATCCCTGGCTGCGGCAAGGTGTATGGCAAGGCTTCGCACCTGAAGGCCCACTTGCGCTGG CACACAGGCGAGAGGCCCTTCGTCTGCAACTGGCTCTTCTGCGGCAAGAGGTTCACTCGTTCGGATGAG CTGGAGCGTCATGTGCGCACTCACACCCGGGAGAAGAAGTTCACCTGCCTGCTCTGCTCCAAGCGCTTT ACCCGAAGCGACCACCTGAGCAAACACCAGCGCACCCATGGAGAACCAGGCCCGGGTCCCCCTCCCAGT GGCCCCAAGGAGCTGGGGGAGGGCCGCAGCACGGGGGAAGAGGAGGCCAGTCAGACGCCCCGACCTTCT GCCTCGCCAGCAACCCCAGAGAAAGCCCCTGGAGGCAGCCCTGAGCAGAGCAACTTGCTGGAGATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300837
Insert Size	1242 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300837.1
RefSeq Size	3211 bp
RefSeq ORF	1242 bp
Locus ID	121340
UniProt ID	Q8TDD2
Protein Families	ES Cell Differentiation/IPS, Transcription Factors
MW	43.1 kDa
Gene Summary	This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010] Transcript Variant: This variant (3) differs in the 5' structure, resulting in the use of a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a.

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