TMEM117 (NM_001286212) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335940 representing NM_001286212. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTACTTGCCATTCTCACAGGACTAATAGCTGGCAAATTTCTGTTCCATCAGCGTTTGTTTGGTCA GTTGCTCCGATTAAAAATGTTTCGAGAAGATCATGGGTCGTGGATGACAATGTTCTTCAGCACAATTCT CTTTCTCTTCATATTTTCTCACATATACAACACGATTCTTCTAATGGATGGGAACATGGGGTCACTGAT ATGATGCTTCAGGACAAACCCTATCCTGACTGGGGAAAATCAGCAAGAGCTTTCTGGAAGAAAGGAAAT GTTAGGATCACTTTATTCTGGACAGTTCTTTTTACTCTGACGTCTGTGGTTGTACTTGTGATTACAACG GACTGGATCAGCTGGGACAAGCTGAATCGGGGATTTTTGCCCAGTGATGAAGTTTCCAGAGCATTCCTT GCTTCTTTTATCTTGGTCTTTGACCTTCTTATTGTGATGCAGGACTGGGAATTCCCACATTTCATGGGA GATGTTGATGTAAATCTCCCTGGTTTGCACACCCCTCACATGCAGTTCAAGATTCCTTTCTTCCAGAAA ATCTTCAAGGAGGAATATCGTATTCACATAACAGGCAAATGGTTTAACTATGGAATTATCTTCCTCGTC TTGATTTTGGATCTTAATATGTGGAAGAACCAAATATTTTATAAACCTCATGAATATGGGCAATATATC GGCCCGGGGCAGAAGATATATACAGTGAAAGACTCAGAAAGTTTAAAAGATTTGAACAGAACCAAGCTA TCCTGGGAATGGAGGTCCAATCACACTAACCCTCGGACTAATAAAACATATGTTGAGGGAGACATGTTC TTACACAGCAGGTTCATAGGAGCCAGTCTTGATGTCAAGTGTCTGGCCTTTGTTCCAAGCCTGATAGCC TTTGTGTGGTTTGGATTCTTTATTTGGTTCTTTGGACGATTTTTGAAAAATGAGCCACGCATGGAGAAT CAAGACAAAACTTACACTCGCATGAAAAGAAAATCTCCATCAGAACATAGCAAAGACATGGGAATCACT CGAGAAAACACCCAGGCTTCAGTAGAAGACCCCTTGAATGACCCTTCTTTGGTTTGCATCAGGTCTGAC TTCAATGAGATCGTCTACAAGTCTTCCCACCTAACCTCGGAAAACTTGAGCTCACAGTTGAACGAATCT ACTAGTGCAACAGAAGCTGATCAAGACCCAACGACTTCTAAAAGTACACCTACGAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286212
Insert Size	1233 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286212.1
RefSeq Size	2904 bp
RefSeq ORF	1233 bp
Locus ID	84216
Protein Families	Transmembrane
MW	47.8 kDa
Gene Summary	Involved in endoplasmic reticulum (ER) stress-induced cell death pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate exon in the central coding region and initiates translation at an alternate downstream start codon, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a.

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