PPP3CB (NM_001289969) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CALNA2; CALNB; CNA2; PP2Bbeta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335939 representing NM_001289969. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATAGAAGTAGAAGCTCCAATCACAGTGTGTGGTGACATCCATGGCCAATTTTTTGATCTGATGAAA CTTTTTGAAGTAGGAGGATCACCTGCTAATACACGATACCTTTTTCTTGGCGATTATGTGGACAGAGGT TATTTTAGTATAGAGTGTGTCTTATATTTATGGGTTCTGAAGATTCTATACCCAAGCACATTATTTCTT CTGAGAGGCAACCATGAATGCAGACACCTTACTGAATATTTTACCTTTAAGCAGGAATGTAAAATTAAG TATTCGGAAAGAGTCTATGAAGCTTGTATGGAAGCTTTTGATAGTTTGCCTCTTGCTGCACTTTTAAAC CAACAGTTTCTTTGTGTTCATGGTGGACTTTCACCAGAAATACACACACTGGATGATATTAGGAGATTA GATAGATTCAAAGAGCCACCTGCATTTGGACCAATGTGTGACTTGTTATGGTCCGATCCTTCTGAAGAT TTTGGAAATGAAAAATCACAGGAACATTTTAGTCACAATACAGTTCGAGGATGTTCTTATTTTTATAAC TATCCAGCAGTGTGTGAATTTTTGCAAAACAATAATTTGTTATCGATTATTAGAGCTCATGAAGCTCAA GATGCAGGCTATAGAATGTACAGAAAAAGTCAAACTACAGGGTTCCCTTCATTAATAACAATTTTTTCG GCACCTAATTACTTAGATGTCTACAATAATAAAGCTGCTGTATTAAAGTATGAAAATAATGTGATGAAT ATTCGACAGTTTAACTGTTCTCCACATCCTTACTGGTTGCCTAATTTTATGGATGTCTTCACGTGGTCT TTACCGTTTGTTGGAGAAAAAGTGACAGAAATGTTGGTAAATGTTCTGAGTATTTGCTCTGATGATGAA CTAATGACTGAAGGTGAAGACCAGTTTGATGGTTCAGCTGCAGCCCGGAAAGAAATCATAAGAAACAAA ATTCGAGCAATTGGCAAGATGGCAAGAGTCTTCTCTGTTCTCAGGGAGGAGAGTGAAAGTGTGCTGACA CTCAAGGGCCTGACTCCCACAGGGATGTTGCCTAGTGGAGTGTTAGCTGGAGGACGGCAGACCCTGCAA AGTGGTAATGATGTTATGCAACTTGCTGTGCCTCAGATGGACTGGGGCACACCTCACTCTTTTGCTAAC AATTCACATAATGCATGCAGGGAATTCCTTCTGTTTTTTAGTTCCTGTCTCAGCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289969
Insert Size	1233 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289969.1
RefSeq Size	2581 bp
RefSeq ORF	1233 bp
Locus ID	5532
UniProt ID	P16298
Protein Families	Druggable Genome, Phosphatase, Transcription Factors
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
MW	46.7 kDa
Gene Summary	Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:19154138, PubMed:26794871). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (5) has multiple differences in the coding region, one of which results in a frameshift and early stop codon, compared to variant 1. The resulting protein (isoform e) has a distinct C-terminus and is shorter than isoform a.

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