SLC35A2 (NM_001282650) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDG2M; CDGX; UDP-Gal-Tr; UGALT; UGAT; UGT; UGT1; UGT2; UGTL
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335916 representing NM_001282650. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCAGCGGTTGGGGCTGGTGGTTCCACCGCGGCGCCCGGGCCAGGGGCGGTTTCCGCGGGTGCATTG GAGCCGGGGACCGCCAGTGCGGGTGAGACAGTCTGCCCGAGCAGTCGCATGGGAGGCGGAGCTCACAGG CGCCTGAAGTACATATCCCTAGCTGTGCTGGTGGTCCAGAATGCCTCCCTCATCCTCAGCATCCGCTAC GCCCGCACGTTGCCAGGGGACCGCTTCTTTGCCACCACTGCTGTGGTCATGGCGGAAGTGCTCAAAGGT CTCACCTGCCTGCTGCTGCTCTTCGCACAGAAGAGGGGTAACGTGAAGCACCTGGTTCTCTTCCTCCAT GAGGCTGTCCTGGTGCAGTATGTGGACACGCTCAAGCTCGCAGTGCCCTCTCTCATCTACACCTTGCAG AATAACCTCCAGTATGTTGCCATCTCTAACCTACCAGCTGCCACTTTCCAGGTGACATACCAGCTGAAG ATCCTGACCACAGCGCTGTTCTCCGTGCTCATGCTGAATCGCAGCCTTTCCCGGCTGCAGTGGGCCTCC CTGCTGCTCCTCTTCACTGGCGTCGCCATTGTCCAGGCACAGCAAGCCGGTGGGGGAGGCCCACGGCCA CTGGATCAGAACCCTGGGGCAGGCCTGGCAGCCGTCGTGGCCTCCTGTCTCTCCTCCGGCTTCGCAGGT GTCTACTTTGAGAAGATCCTCAAAGGCAGCTCAGGCTCCGTGTGGCTGCGCAACCTGCAACTGGGCCTC TTCGGCACAGCACTGGGCCTGGTGGGGCTCTGGTGGGCTGAGGGTACCGCCGTGGCCACCCGTGGTTTC TTTTTTGGGTACACACCTGCTGTCTGGGGCGTGGTGCTCAACCAGGCCTTCGGCGGGCTACTGGTGGCT GTGGTTGTCAAGTACGCTGACAATATCCTCAAGGGCTTTGCCACCTCCCTGTCCATTGTGCTGTCCACT GTTGCCTCCATTCGCCTCTTTGGCTTCCACGTGGACCCATTATTTGCCCTTGGCGCTGGACTCGTCATT GGTGCTGTCTACCTCTACAGCCTTCCCCGAGGTGCAGCCAAAGCCATAGCCTCTGCCTCTGCCTCCGCC TCCGGGCCCTGCGTTCACCAGCAGCCTCCCGGGCAGCCACCACCACCGCAGCTGTCTTCCCACCGTGGA GACCTCATCACGGAGCCCTTTCTGCCAAAGTCAGTGCTGGTGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282650
Insert Size	1221 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282650.1
RefSeq Size	2658 bp
RefSeq ORF	1221 bp
Locus ID	7355
UniProt ID	P78381
Protein Families	Transmembrane
MW	42.2 kDa
Gene Summary	This gene encodes a member of the nucleotide-sugar transporter family. The encoded protein is a multi-pass membrane protein. It transports UDP-galactose from the cytosol into Golgi vesicles, where it serves as a glycosyl donor for the generation of glycans. Mutations in this gene cause congenital disorder of glycosylation type IIm (CDG2M). Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (7) uses an alternate in-frame splice site in the 5' coding region and differs in the 3' exon structure, compared to variant 1. The encoded isoform (g) is longer and has a distinct C-terminus, compared to isoform a.

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