ALDH1A3 (NM_001293815) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ALDH1A6; ALDH6; MCOP8; RALDH3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335910 representing NM_001293815. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCACCGCTAACGGGGCCGTGGAAAACGGGCAGCCGGACAGGAAGCCGCCGGCCCTGCCGCGCCCC ATCCGCAACCTGGAGGTCAAGTTCACCAAGATATTTATCAACAATGAATGGCACGAATCCAAGAGTGGG AAAAAGTTTGCTACATGTAACCCTTCAACTCGGGAGCAAATATGTGAAGTGGAAGAAGGAGATAAGCCC GACGTGGACAAGGCTGTGGAGGCTGCACAGGTTGCCTTCCAGAGGGGCTCGCCATGGCGCCGGCTGGAT GCCCTGAGTCGTGGGCGGCTGCTGCACCAGCTGGCTGACCTGGTGGAGAGGGACCGCGCCACCTTGGCC GCCGGGTTCCCTCCAGGAGTGGTGAACATTGTGCCAGGATTCGGGCCCACAGTGGGAGCAGCAATTTCT TCTCACCCTCAGATCAACAAGATCGCCTTCACCGGCTCCACAGAGGTTGGAAAACTGGTTAAAGAAGCT GCGTCCCGGAGCAATCTGAAGCGGGTGACGCTGGAGCTGGGGGGGAAGAACCCCTGCATCGTGTGTGCG GACGCTGACTTGGACTTGGCAGTGGAGTGTGCCCATCAGGGAGTGTTCTTCAACCAAGGCCAGTGTTGC ACGGCAGCCTCCAGGGTGTTCGTGGAGGAGCAGGTCTACTCTGAGTTTGTCAGGCGGAGCGTGGAGTAT GCCAAGAAACGGCCCGTGGGAGACCCCTTCGATGTCAAAACAGAACAGGGGCCTCAGATTGATCAAAAG CAGTTCGACAAAATCTTAGAGCTGATCGAGAGTGGGAAGAAGGAAGGGGCCAAGCTGGAATGCGGGGGC TCAGCCATGGAAGACAAGGGGCTCTTCATCAAACCCACTGTCTTCTCAGAAGTCACAGACAACATGCGG ATTGCCAAAGAGGAGATTTTCGGGCCAGTGCAACCAATACTGAAGTTCAAAAGTATCGAAGAAGTGATA AAAAGAGCGAATAGCACCGACTATGGACTCACAGCAGCCGTGTTCACAAAAAATCTCGACAAAGCCCTG AAGTTGGCTTCTGCCTTAGAGTCTGGAACGGTCTGGATCAACTGCTACAACGCCCTCTATGCACAGGCT CCATTTGGTGGCTTTAAAATGTCAGGAAATGGCAGAGAACTAGGTGAATACGCTTTGGCCGAATACACA GAAGTGAAAACTGTCACCATCAAACTTGGCGACAAGAACCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001293815
Insert Size	1218 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293815.1
RefSeq Size	3301 bp
RefSeq ORF	1218 bp
Locus ID	220
UniProt ID	P47895
Protein Families	Druggable Genome
Protein Pathways	Drug metabolism - cytochrome P450, Glycolysis / Gluconeogenesis, Histidine metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Phenylalanine metabolism, Tyrosine metabolism
MW	44.3 kDa
Gene Summary	This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] Transcript Variant: This variant (2) lacks three alternate in-frame exons, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

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