ARFGAP1 (NM_001281482) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARF1GAP; HRIHFB2281
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335899 representing NM_001281482. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCAGCCCAAGAACCAGGAAGGTTCTTAAAGAAGTCAGGGTGCAGGATGAGAACAACGTTTGTTTT GAGTGTGGCGCGTTCAATCCTCAGTGGGTCAGTGTGACCTACGGCATCTGGATCTGCCTGGAGTGCTCG GGGAGACACCGCGGGCTTGGGGTTCACCTCAGCTTTGTGCGCTCTGTTACTATGGACAAGTGGAAGGAC ATTGAGCTTGAGAAGATGAAAGCTGGTGGGAATGCTAAGTTCCGAGAGTTCCTGGAGTCTCAGGAGGAT TACGATCCTTGCTGGTCCTTGCAGGAGAAGTACAACAGCAGAGCCGCGGCCCTCTTTAGGGATAAGGTG GTCGCTCTGGCCGAAGGCAGAGAGTGGTCTCTGGAGTCATCACCTGCCCAGAACTGGACCCCACCTCAG CCCAGGACGCTGCCGTCCATGGTGCACCGAGTCTCTGGCCAGCCGCAGAGTGTGACCGCCTCCTCGGAC AAGGCTTTTGAAGACTGGCTGAATGATGACCTCGGCTCCTATCAAGGGGCCCAGGGGAATCGCTACGTG GGGTTTGGGAACACGCCACCGCCTCAGAAGAAAGAAGATGACTTCCTCAACAACGCCATGTCCTCCCTG TACTCGGGCTGGAGCAGCTTCACCACTGGAGCCAGCCGGTTTGCCTCGGCAGCCAAGGAGGGCGCTACA AAGTTTGGATCCCAAGCGAGTCAGAAGGCGTCCGAGCTGGGCCACAGCCTGAACGAGAACGTCCTCAAG CCTGCGCAGGAGAAGGTGAAGGAGGGAAAGATTTTTGATGATGTCTCCAGTGGGGTCTCTCAGTTGGCG TCCAAGTGCCAGCGCCGTCTTTGCTGCCATCAGTCCCACTGCTCTGCGGGCCATTTGGGGCGTGCATTT TGTCCTGTTTCCTGGCATGAGGCGCTCTGCGGACAGACGGGGAGGGAAGAGCAGGCCTCGCTCCTCCCC CCCAAGCATGTGGTGGGAGCTCTTGAGGTCTGTGCACGAGGCTGTCCTCGCTGCCATGTCCCGCACACA CCTGGCACCGCTGCAGAGTGGCCGGGGCGTCTGTGTCTGTCCAGGGAGTCGGTAGTAAGGGATGGCGGG ACGTCACCACCTTTTTTTCGGGGAAAGCAGAGGGCCCCTTGGACAGCCCCTCGGAGGGCCACAGTTATC AGAACAGCGGTCTGGACCACTTCCAAAACAGCAACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281482
Insert Size	1212 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281482.1
RefSeq Size	3524 bp
RefSeq ORF	1212 bp
Locus ID	55738
UniProt ID	Q8N6T3
Protein Pathways	Endocytosis
MW	44.5 kDa
Gene Summary	The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) has multiple differences in the coding region, which results in a translational frameshift, compared to variant 2. The encoded isoform (c) has a shorter and distinct C-terminus, compared to isoform b.

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