PRUNE (PRUNE1) (NM_001303242) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DRES-17; DRES17; H-PRUNE; HTCD37; NMIHBA; PRUNE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335877 representing NM_001303242. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGACTACCTGCAGGGTTGTCGAGCTGCTCTGCAGGAGTCCCGACCTCTACATGTTGTGCTGGGA AATGAAGCCTGTGATTTGGACTCCACAGTGTCTGCTCTTGCCCTGGCTTTTTACCTAGCAAAGACAACT GAGGCTGAGGAAGTCTTTGTGCCAGTTTTAAATATAAAACGTTCTGAACTACCTCTGCGAGGTGACATT GTCTTCTTTCTTCAGAAGGTTCATATTCCAGAGAGTATCTTGATTTTTCGGGATGAGATTGACCTCCAT GCATTATACCAGGCTGGCCAACTCACCCTCATCCTTGTCGACCATCATATCTTATCCAAAAGTGACACA GCCCTAGAGGAGGCAGTAGCAGAGGTGCTAGACCATCGACCCATCGAGCCGAAACACTGCCCTCCCTGC CATGTTTCAGTTGAGCTGGTGGGGTCCTGTGCTACCCTGGTGACCGAGAGAATCCTGCAGGGGGCACCA GAGATCTTGGACAGGCAAACTGCAGCCCTTCTGCATGGAACCATCATCCTGGACTGTGTCAACATGGAC CTTAAAATTGGAAAGGCAACCCCAAAGGACAGCAAATATGTGGAGAAACTAGAGGCCCTTTTCCCAGAC CTACCCAAGAGAAATGATATATTTGATTCCCTACAAAAGGCAAAGTTTGATGTATCAGGACTGACCACT GAGCAGATGCTGAGAAAAGACCAGAAGACTATCTATAGACAAGGCGTCAAGGTGGCCATTAGTGCAATA TATATGGATTTGGAGATCTGTGAAGTCCTGGAACGCTCCCACTCTCCACCCCTGAAGCTGACCCCTGCC TCAAGTACCCACCCTAACCTCCATGCCTATCTTCAAGGCAACACCCAGGTCTCTCGAAAGAAACTTCTG CCCCTGCTCCAGGAAGCCCTGTCAGCATATTTTGACTCCATGAAGATCCCTTCAGGACAGCCTGAGACA GCAGATGTGTCCAGGGAGCAAGTGGACAAGGAATTGGACAGGGCAAGTAACTCCCTGATTTCTGGCCTG AGTCAAGATGAGGAGGACCCTCCGCTGCCCCCGACGCCCATGAACAGCTTGGTGGATGAGTGCCCTCTA GATCAGGGGCTGCCTAAACTCTCTGCTGAGGCCGTCTTCGAGAAGTGCAGTCAGATCTCACTGTCACAG TCTACCACAGCCTCCCTGTCCAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001303242
Insert Size	1203 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303242.1
RefSeq Size	2942 bp
RefSeq ORF	1203 bp
Locus ID	58497
UniProt ID	Q86TP1
Protein Pathways	Purine metabolism
MW	44.2 kDa
Gene Summary	This gene encodes a member of the DHH protein superfamily of phosphoesterases. This protein has been found to function as both a nucleotide phosphodiesterase and an exopolyphosphatase. This protein is believed to stimulate cancer progression and metastases through the induction of cell motility. A pseuodgene has been identified on chromosome 13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 1. The resulting protein (isoform 3) is shorter than isoform 1.

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