MTERF (MTERF1) (NM_001301134) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MTERF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335746 representing NM_001301134. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCACCAGGAAACCTCTGGCATATGAGAAATAACTTTCTCTTTGGTTCAAGATGTTGGATGACTCGA TTTTCAGCAGAAAACATCTTCAAATCAGTTTCATTTAGGCTTTTTGGTGTGAAGTGTCATAATACAGAC AGTGAGCCTTTGAAAAATGAGGACCTACTGAAAAACTTACTTACTATGGGAGTAGATATTGACATGGCA AGGAAACGACAGCCTGGAGTTTTTCATAGGATGATTACCAATGAGCAGGACCTGAAGATGTTCCTTCTT TCCAAAGGAGCTAGCAAAGAAGTGATCGCTAGCATCATATCAAGATATCCACGAGCAATAACACGTACT CCCGAGAATCTTTCAAAACGGTGGGATCTGTGGAGAAAGATTGTGACATCAGACCTTGAAATTGTAAAT ATTTTGGAACGTTCTCCTGAATCCTTTTTTCGGTCCAATAACAACCTAAACTTAGAGAATAATATAAAG TTCCTCTACTCAGTTGGATTGACCCGTAAATGCCTTTGTCGATTGTTGACCAATGCCCCTCGTACCTTC TCCAATAGTCTTGATCTGAATAAACAGATGGTTGAATTTTTGCAGGCAGCCGGTTTGTCATTGGGTCAC AATGATCCCGCAGATTTTGTCAGAAAGATAATTTTTAAAAACCCTTTTATCTTAATTCAGAGCACCAAG CGGGTGAAAGCTAACATTGAATTCTTACGGTCAACTTTCAATTTGAACAGTGAGGAACTGCTGGTTCTG ATATGTGGTCCAGGAGCTGAAATCCTAGACCTTTCCAATGACTATGCCAGAAGAAGCTACGCAAACATC AAAGAGAAGCTGTTTTCTCTTGGATGTACTGAAGAAGAGGTACAGAAGTTTGTCTTAAGCTATCCAGAT GTGATCTTCTTGGCAGAGAAAAAGTTTAATGATAAAATAGACTGCCTCATGGAAGAAAACATTAGCATT TCACAAATAATCGAAAATCCTCGGGTTCTGGATTCAAGCATAAGTACTTTAAAAAGTCGAATCAAAGAA TTGGTAAATGCTGGCTGTAACTTGAGTACTTTAAACATCACTCTTCTATCTTGGAGTAAAAAAAGATAT GAAGCTAAATTGAAAAAGTTAAGCAGATTTGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301134
Insert Size	1140 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301134.1
RefSeq Size	1968 bp
RefSeq ORF	1140 bp
Locus ID	7978
UniProt ID	Q99551
Protein Families	Transcription Factors
MW	43.6 kDa
Gene Summary	This gene encodes a mitochondrial transcription termination factor. This protein participates in attenuating transcription from the mitochondrial genome; this attenuation allows higher levels of expression of 16S ribosomal RNA relative to the tRNA gene downstream. The product of this gene has three leucine zipper motifs bracketed by two basic domains that are all required for DNA binding. There is evidence that, for this protein, the zippers participate in intramolecular interactions that establish the three-dimensional structure required for DNA binding. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks part of the 5' coding region and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Both variants 2 and 3 encode the same isoform.

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