VPS36 (NM_001282168) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C13orf9; CGI-145; EAP45
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335737 representing NM_001282168. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACCGCTTCGTTTGGACCAGCGGCCTCCTGGAGATCAACGAGACCCTGGTGATCCAGCAGCGCGGG ATAAAATTTGATGCTGGGACTCTCCTTCTTAGTACACACCGACTGATTTGGAGAGATCAGAAAAATCAT GAGTGTTGCATGGCCATTCTCCTTTCCCAAATTGTGTTCATTGAAGAACAGGCGGCTGGAATTGGGAAG AGTGCCAAAATAGTGGTTCATCTTCACCCAGCTCCTCCTAACAAAGAACCTGGCCCATTCCAGAGTAGT AAGAACTCCTACATCAAACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAG GAAATGACACAAAGAAGATGGGAGAATATGCCAGTTTCCCAGTCATTACAAACAAATAGAGGACCCCAG CCAGGAAGAATAAGGGCTGTAGGAATTGTAGGTATTGAAAGGAAACTGGAAGAAAAAAGAAAAGAAACT GACAAAAACATTTCTGAGGCCTTTGAAGACCTCAGCAAACTAATGATCAAGGCTAAGGAAATGGTGGAA TTATCAAAATCAATTGCTAATAAAATTAAAGACAAACAAGGTGACATCACAGAAGATGAGACCATCAGG TTTAAATCCTACTTGCTGAGCATGGGAATAGCTAACCCAGTTACCAGAGAAACCTACGGCTCAGGCACA CAGTACCACATGCAGCTGGCCAAACAACTGGCTGGAATATTGCAGGTGCCTTTAGAGGAACGAGGGGGA ATAATGTCACTCACGGAGGTGTACTGCTTAGTAAACCGAGCTCGAGGAATGGAATTGCTCTCACCAGAA GATTTAGTGAATGCGTGCAAGATGCTGGAAGCACTGAAATTACCTCTCAGGCTCCGTGTGTTTGACAGT GGCGTCATGGTAATTGAGCTTCAGTCTCACAAGGAAGAGGAAATGGTGGCCTCGGCCCTGGAGACAGTT TCAGAAAAGGGATCCCTAACATCAGAAGAGTTTGCTAAGCTTGTGGGAATGTCTGTCCTCCTAGCCAAA GAAAGGTTGCTGCTTGCAGAGAAGATGGGCCATCTTTGCCGTGATGACTCAGTGGAAGGCCTGCGTTTT TACCCAAATTTATTTATGACACAGAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282168
Insert Size	1134 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282168.1
RefSeq Size	4446 bp
RefSeq ORF	1134 bp
Locus ID	51028
Protein Families	Transcription Factors
Protein Pathways	Endocytosis
MW	42.7 kDa
Gene Summary	This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' terminal exon compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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