ULK3 (NM_001284365) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335579 representing NM_001284365. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAGCAATTAGCTAGCGCCCTGCAATTCCTGCATGAACGGAATATCTCTCACCTGGATCTGAAGCCA CAGAACATTCTACTGAGCTCCTTGGAGAAGCCCCACCTAAAACTGGCAGACTTTGGTTTCGCACAACAC ATGTCCCCGTGGGATGAGAAGCACGTGCTCCGTGGCTCCCCCCTCTACATGGCCCCCGAGATGGTGTGC CAGCGGCAGTATGACGCCCGCGTGGACCTCTGGTCCATGGGGGTCATCCTGTATGAAGCCCTCTTCGGG CAGCCCCCCTTTGCCTCCAGGTCGTTCTCGGAGCTGGAAGAGAAGATCCGTAGCAACCGGGTCATCGAG CTCCCCTTGCGGCCCCTGCTCTCCCGAGACTGCCGGGACCTACTGCAGCGGCTCCTGGAGCGGGACCCC AGCCGTCGCATCTCCTTCCAGGACTTTTTTGCGCACCCCTGGGTGGACCTGGAGCACATGCCCAGTGGG GAGAGTCTGGGGCGAGCAACCGCCCTGGTGGTGCAGGCTGTGAAGAAAGACCAGGAGGGGGATTCAGCA GCTGCCTTATCACTCTACTGCAAGGCTCTGGACTTCTTTGTACCTGCCCTGCACTATGAAGTGGATGCC CAGCGGAAGGAGGCAATTAAGGCAAAGGTGGGGCAGTACGTGTCCCGGGCTGAGGAGCTCAAGGCCATC GTCTCCTCTTCCAATCAGGCCCTGCTGAGGCAGGGGACCTCTGCCCGAGACCTGCTCAGAGAGATGGCC CGGGACAAGCCACGCCTCCTAGCTGCCCTGGAAGTGGCTTCAGCTGCCATGGCCAAGGAGGAGGCCGCC GGCGGGGAGCAGGATGCCCTGGACCTGTACCAGCACAGCCTGGGGGAGCTACTGCTGTTGCTGGCAGCG GAGCCCCCGGGCCGGAGGCGGGAGCTGCTTCACACTGAGGTTCAGAACCTCATGGCCCGAGCTGAATAC TTGAAGGAGCAGGTCAAGATGAGGGAATCTCGCTGGGAAGCTGACACCCTGGACAAAGAGGGACTGTCG GAATCTGTTCGTAGCTCTTGCACCCTTCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001284365
Insert Size	1068 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284365.2
RefSeq Size	2567 bp
RefSeq ORF	1068 bp
Locus ID	25989
UniProt ID	Q6PHR2
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	mTOR signaling pathway, Regulation of autophagy
MW	40.2 kDa
Gene Summary	Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site in the 5' region and initiates translation from a downstream in-frame start codon, compared to variant 1. The encoded isoform (c) is shorter than isoform a.

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