DTX3 (NM_001286245) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	deltex3; RNF154
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335539 representing NM_001286245. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCAATTCTAAGCTCTTCAGGATCAAAAATGGCAGCCTGTGGAGGCACCTGCAAGAACAAAGTGACT GTGTCCAAGCCCGTGTGGGACTTCCTGAGCAAAGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAG CACCGTGTGTCCATCCTCATAGATGGCGAGACTTCTGACATCTATGTTCTCCAGCTTTCCCCACAGGGT CCTCCCCCGGCCCCTCCAAATGGGCTCTACCTAGCCCGGAAGGCTCTCAAGGGGCTGCTAAAAGAGGCA GAGAAAGAGCTGAAGAAAGCTCAGAGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGGG GAGCACCCTGAGATGCACCGCGCAGGCCCACCCCCTCTCCGAGCAGCCCCACTTCTGCCCCCAGGAGCT CGGGGGCTCCCCCCTCCTCCTCCCCCCCTGCCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCAGAA GAGCAGGAGAGCACCTGCCCCATCTGTCTGGGGGAGATCCAGAATGCCAAGACATTGGAGAAGTGCCGG CATTCATTCTGCGAGGGCTGCATCACCCGGGCTCTGCAGGTGAAAAAGGCCTGCCCCATGTGCGGCCGC TTCTATGGGCAGCTGGTGGGCAACCAGCCCCAGAATGGGCGGATGCTGGTCTCTAAGGACGCCACCCTC CTACTGCCCAGCTATGAGAAGTACGGCACCATTGTCATCCAGTACGTCTTCCCGCCCGGTGTCCAGGGG GCTGAACACCCAAACCCAGGAGTTCGGTATCCTGGCACCACACGGGTGGCCTACCTCCCGGACTGCCCT GAGGGCAACAAGGTGCTGACCCTGTTCCGCAAGGCGTTTGACCAGCGTCTCACCTTCACTATCGGCACG TCCATGACCACAGGGAGACCGAATGTCATCACCTGGAACGACATCCACCACAAGACCAGCTGCACAGGG GGACCCCAGCTGTTTGGGTACCCAGACCCCACCTACCTGACCCGGGTGCAAGAGGAGCTGAGAGCGAAG GGTATCACAGATGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286245
Insert Size	1053 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286245.1
RefSeq Size	2062 bp
RefSeq ORF	1053 bp
Locus ID	196403
UniProt ID	Q8N9I9
Protein Families	Druggable Genome
Protein Pathways	Notch signaling pathway
MW	38.2 kDa
Gene Summary	DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009] Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR, uses an alternate splice site in the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is longer than isoform a.

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