DTX3 (NM_001286245) Human Untagged Clone

CAT#: SC335539

DTX3 (untagged) - Human deltex 3, E3 ubiquitin ligase (DTX3), transcript variant 3



  "NM_001286245" in other vectors (2)

CNY 3,420.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
Anti-DTX3 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms deltex3; RNF154
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC335539 representing NM_001286245.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCAATTCTAAGCTCTTCAGGATCAAAAATGGCAGCCTGTGGAGGCACCTGCAAGAACAAAGTGACT
GTGTCCAAGCCCGTGTGGGACTTCCTGAGCAAAGAGACCCCAGCCCGGCTGGCCCGGCTTCGGGAGGAG
CACCGTGTGTCCATCCTCATAGATGGCGAGACTTCTGACATCTATGTTCTCCAGCTTTCCCCACAGGGT
CCTCCCCCGGCCCCTCCAAATGGGCTCTACCTAGCCCGGAAGGCTCTCAAGGGGCTGCTAAAAGAGGCA
GAGAAAGAGCTGAAGAAAGCTCAGAGGCAGGGGGAGCTGATGGGCTGCCTGGCTCTGGGGGGTGGAGGG
GAGCACCCTGAGATGCACCGCGCAGGCCCACCCCCTCTCCGAGCAGCCCCACTTCTGCCCCCAGGAGCT
CGGGGGCTCCCCCCTCCTCCTCCCCCCCTGCCCCCACCTCTTCCTCCTCGCCTTCGGGAGGAGGCAGAA
GAGCAGGAGAGCACCTGCCCCATCTGTCTGGGGGAGATCCAGAATGCCAAGACATTGGAGAAGTGCCGG
CATTCATTCTGCGAGGGCTGCATCACCCGGGCTCTGCAGGTGAAAAAGGCCTGCCCCATGTGCGGCCGC
TTCTATGGGCAGCTGGTGGGCAACCAGCCCCAGAATGGGCGGATGCTGGTCTCTAAGGACGCCACCCTC
CTACTGCCCAGCTATGAGAAGTACGGCACCATTGTCATCCAGTACGTCTTCCCGCCCGGTGTCCAGGGG
GCTGAACACCCAAACCCAGGAGTTCGGTATCCTGGCACCACACGGGTGGCCTACCTCCCGGACTGCCCT
GAGGGCAACAAGGTGCTGACCCTGTTCCGCAAGGCGTTTGACCAGCGTCTCACCTTCACTATCGGCACG
TCCATGACCACAGGGAGACCGAATGTCATCACCTGGAACGACATCCACCACAAGACCAGCTGCACAGGG
GGACCCCAGCTGTTTGGGTACCCAGACCCCACCTACCTGACCCGGGTGCAAGAGGAGCTGAGAGCGAAG
GGTATCACAGATGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001286245
Insert Size 1053 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001286245.1
RefSeq Size 2062 bp
RefSeq ORF 1053 bp
Locus ID 196403
UniProt ID Q8N9I9
Protein Families Druggable Genome
Protein Pathways Notch signaling pathway
MW 38.2 kDa
Gene Summary DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009]
Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR, uses an alternate splice site in the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is longer than isoform a.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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