Adenylate cyclase 1 (ADCY1) (NM_001281768) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AC1; DFNB44
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335456 representing NM_001281768. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTATGGGGTCTTTGTGCGGATTCTGACTGAGCGTTCACAGAGGAAGGCGTTCCTGCAGGCCCGGAGC TGCATTGAGGACCGACTGAGGCTGGAGGATGAGAACGAGAAGCAGGAGCGGCTCCTCATGAGCCTCCTG CCCCGGAACGTTGCCATGGAGATGAAGGAGGACTTCCTGAAGCCCCCTGAGAGGATTTTCCACAAGATT TACATCCAGAGGCACGACAATGTGAGCATCCTGTTTGCTGACATCGTGGGTTTCACGGGCTTGGCATCC CAGTGCACAGCCCAGGAGCTGGTGAAACTCCTCAATGAGCTCTTCGGCAAGTTCGATGAATTAGCCACG GAGAACCACTGTCGCCGCATCAAGATTCTCGGGGACTGCTACTACTGCGTGTCGGGCCTCACCCAGCCC AAGACTGACCATGCCCACTGCTGTGTGGAGATGGGACTCGACATGATTGATACCATCACATCTGTGGCT GAAGCCACCGAGGTGGATCTGAACATGCGTGTGGGTCTGCACACGGGCAGGGTCCTCTGTGGTGTCCTG GGCTTGCGCAAGTGGCAGTACGACGTGTGGTCCAATGATGTGACCTTGGCCAATGTCATGGAAGCCGCT GGCCTGCCAGGGAAGGTTCATATCACAAAGACGACCCTAGCGTGCTTGAATGGGGACTACGAGGTAGAA CCGGGTTACGGACATGAGAGGAACAGTTTCTTGAAAACTCATAACATCGAAACCTTTTTTATTGTGCCA TCCCATCGCCGAAAGATATTTCCAGGCCTGATTCTCTCAGATATAAAACCGGCCAAAAGGATGAAGTTC AAGACTGTCTGCTACCTGCTGGTGCAGCTCATGCACTGCCGGAAAATGTTCAAGGCCGAGATCCCCTTC TCCAATGTCATGACCTGCGAGGACGATGACAAGGTTCAGATCCATCCCTCACTGAGTGGCCTGGATGTC CTCCTGGGGTTTTGGTCTCCCACCCTGCAACGGGGACGACTGTGCACGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281768
Insert Size	1017 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281768.1
RefSeq Size	1763 bp
RefSeq ORF	1017 bp
Locus ID	107
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Calcium signaling pathway, Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Long-term potentiation, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Vascular smooth muscle contraction
MW	38.7 kDa
Gene Summary	This gene encodes a member of the of adenylate cyclase gene family that is primarily expressed in the brain. This protein is regulated by calcium/calmodulin concentration and may be involved in brain development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (2) differs in the 5' and 3' UTR and has multiple differences in the coding region compared to variant 1. The encoded isoform (2) has a shorter N-terminus and a distinct C-terminus, compared to isoform 1.

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