VPS36 (NM_001282169) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C13orf9; CGI-145; EAP45
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335370 representing NM_001282169. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCATTCTCCTTTCCCAAATTGTGTTCATTGAAGAACAGGCGGCTGGAATTGGGAAGAGTGCCAAA ATAGTGGTTCATCTTCACCCAGCTCCTCCTAACAAAGAACCTGGCCCATTCCAGAGTAGTAAGAACTCC TACATCAAACTCTCCTTCAAAGAACATGGCCAGATTGAGTTTTACAGGCGTTTATCAGAGGAAATGACA CAAAGAAGATGGGAGAATATGCCAGTTTCCCAGTCATTACAAACAAATAGAGGACCCCAGCCAGGAAGA ATAAGGGCTGTAGGAATTGTAGGTATTGAAAGGAAACTGGAAGAAAAAAGAAAAGAAACTGACAAAAAC ATTTCTGAGGCCTTTGAAGACCTCAGCAAACTAATGATCAAGGCTAAGGAAATGGTGGAATTATCAAAA TCAATTGCTAATAAAATTAAAGACAAACAAGGTGACATCACAGAAGATGAGACCATCAGGTTTAAATCC TACTTGCTGAGCATGGGAATAGCTAACCCAGTTACCAGAGAAACCTACGGCTCAGGCACACAGTACCAC ATGCAGCTGGCCAAACAACTGGCTGGAATATTGCAGGTGCCTTTAGAGGAACGAGGGGGAATAATGTCA CTCACGGAGGTGTACTGCTTAGTAAACCGAGCTCGAGGAATGGAATTGCTCTCACCAGAAGATTTAGTG AATGCGTGCAAGATGCTGGAAGCACTGAAATTACCTCTCAGGCTCCGTGTGTTTGACAGTGGCGTCATG GTAATTGAGCTTCAGTCTCACAAGGAAGAGGAAATGGTGGCCTCGGCCCTGGAGACAGTTTCAGAAAAG GGATCCCTAACATCAGAAGAGTTTGCTAAGCTTGTGGGAATGTCTGTCCTCCTAGCCAAAGAAAGGTTG CTGCTTGCAGAGAAGATGGGCCATCTTTGCCGTGATGACTCAGTGGAAGGCCTGCGTTTTTACCCAAAT TTATTTATGACACAGAGCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282169
Insert Size	987 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282169.1
RefSeq Size	4607 bp
RefSeq ORF	987 bp
Locus ID	51028
UniProt ID	Q86VN1
Protein Families	Transcription Factors
Protein Pathways	Endocytosis
MW	37 kDa
Gene Summary	This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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