ZDHHC16 (NM_001287804) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APH2; DHHC-16
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335241 representing NM_001287804. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGAGGCCAGCGGAGCCTGCTGCTGGGCCCGGCCCGCCTCTGCCTCCGCCTCCTTCTGCTGCTGGGT TACAGGCGCCGCTGTCCACCTCTACTCCGGGGTCTAGTACAGCGCTGGCGCTACGGCAAGGTCTGCCTG CGCTCCCTGCTCTACAACTCCTTTGGGGGCAGTGACACCGCTGTTGATGCTGCCTTTGAGCCTGTCTAC TGGCTGGTAGACAACGTGATCCGCTGGTTTGGAGTGGTGTTCGTGGTCCTGGTGATCGTGCTGACAGGC TCCATTGTAGCTATCGCCTACCTGTGTGTCCTGCCTCTCATCCTCCGAACCTACTCAGTGCCACGACTC TGCTGGCATTTCTTCTATAGCCACTGGAATCTGATCCTGATTGTCTTCCACTACTACCAGGCCATCACC ACTCCGCCTGGGTACCCACCCCAGGGCAGGAATGATATCGCCACCGTCTCCATCTGTAAGAAGTGCATT TACCCCAAGCCAGCCCGAACACACCACTGCAGCATCTGCAACAGGTGTGTGCTGAAGATGGATCACCAC TGCCACATACTTCCTCCAGCCCTCTCTAAACTACCCACCAGTCTTCGCCCCTCTTTTCTTAGTTCTGTG GCACTTGCCCTGGGTGCCCTAACTGTATGGCATGCTGTTCTCATCAGTCGAGGTGAGACTAGCATCGAA AGGCACATCAACAAGAAGGAGAGACGTCGGCTACAGGCCAAGGGCAGAGTATTTAGGAATCCTTACAAC TACGGCTGCTTGGACAACTGGAAGGTATTCCTGGGTGTGGATACAGGAAGGCACTGGCTTACTCGGGTG CTCTTACCTTCTAGTCACTTGCCCCATGGGAATGGAATGAGCTGGGAGCCCCCTCCCTGGGTGACTGCT CACTCAGCCTCTGTGATGGCAGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287804
Insert Size	924 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287804.1
RefSeq Size	1646 bp
RefSeq ORF	924 bp
Locus ID	84287
UniProt ID	Q969W1
Protein Families	Transmembrane
MW	35 kDa
Gene Summary	Palmitoyl acyltransferase that mediates palmitoylation of proteins such as PLN and ZDHHC6 (PubMed:28826475). Required during embryonic heart development and cardiac function, possibly by mediating palmitoylation of PLN, thereby affecting PLN phosphorylation and homooligomerization (By similarity). Also required for eye development (By similarity). Palmitoylates ZDHHC6, affecting the quaternary assembly of ZDHHC6, its localization, stability and function (PubMed:28826475). May play a role in DNA damage response (By similarity). May be involved in apoptosis regulation (By similarity). Involved in the proliferation of neural stem cells by regulating the FGF/ERK pathway (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (7) lacks three exons in the central and 3' coding regions and uses an alternate in-frame splice site in the 3' coding region, compared to variant 5. It encodes isoform 6, which is shorter than isoform 1.

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