RITA (RITA1) (NM_001286215) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C12orf52; RITA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286215, the custom clone sequence may differ by one or more nucleotides ATGCTCAGGGAGCCTCGGAAGCAGGGCCTGGCCGGCAGAGCACACCTGCTGTCACCAGGGACCACAGGCA GCATGAAGACCCCCGTGGAGCTGGCCGTCAGTGGGATGCAGACCCTCGGCCTTCAGCACCGCTGCCGAGG TGGCTACCGGGTCAAGGCCAGGACGTCATATGTGGATGAGACTCTGTTTGGCAGCCCAGCAGGCACCCGG CCTACCCCACCGGACTTCGATCCGCCCTGGGTGGAGAAGGCTAACAGAACCAGAGGCGTGGGCAAGGAGG CATCGAAGGCCTTGGGGGCAAAGGGGAGCTGTGAGACCACCCCCTCAAGGGGCAGCACCCCCACCCTCAC ACCAAGGAAGAAGAACAAATACAGACCCATCAGCCACACCCCGTCTTACTGTGATGAGTCGCTGTTTGGC TCCCGATCTGAAGGCGCCAGCTTCGGGGCCCCGCGGATGGCGAAGGGGGATGCCGCAAAGCTCCGTGCTC TCTTGTGGACGCCACCACCTACCCCCAGGGGTAGCCACTCGCCCCGCCCCAGGGAGGCACCACTGCGAGC CATTCACCCAGCTGGTCCCTCCAAGACAGAGCCGGGGCCAGCGGCAGACTCCCAGAAGTTATCTATGGGT GGGTTACACTCTTCACGCCCCCTGAAGCGGGGACTTTCCCATTCCCTCACCCACCTGAATGTCCCCAGCA CTGGTCATCCAGCCACCAGTGCCCCCCACACAAATGGGCCTCAGGATCTCAGGCCTTCCACGTCAGGGGT GACCTTCCGGAGCCCCCTGGTGACTTCCAGGGCTCGCTCAGTTAGCATTTCAGTGCCATCTACCCCACGA CGAGGTGGGGCCACCCAGAAACCAAAGCCCCCTTGGAAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286215
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286215.1, NP_001273144.1
RefSeq Size	1921 bp
RefSeq ORF	882 bp
Locus ID	84934
UniProt ID	Q96K30
Gene Summary	Tubulin-binding protein that acts as a negative regulator of Notch signaling pathway. Shuttles between the cytoplasm and the nucleus and mediates the nuclear export of RBPJ/RBPSUH, thereby preventing the interaction between RBPJ/RBPSUH and NICD product of Notch proteins (Notch intracellular domain), leading to down-regulate Notch-mediated transcription. May play a role in neurogenesis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate exon in the 5' region and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.