PPP2R2D (NM_001291310) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	B55D; B55delta; MDS026
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291310, the custom clone sequence may differ by one or more nucleotides ATGGATCTTATGGTAGAAGCGAGTCCACGGCGAATTTTTGCAAATGCTCACACATATCATATAAATTCCA TTTCAGTAAATAGTGATCATGAAACATATCTTTCTGCAGATGACCTGAGAATTAATTTATGGCACTTAGA AATCACAGATAGAAGCTTTAACATCGTGGACATCAAGCCTGCTAACATGGAGGAGCTGACCGAAGTCATC ACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTGTTCGTCTACAGCAGTAGCAAAGGGACCATCCGCC TGTGTGACATGCGCTCCTCGGCCCTGTGCGACAGACACTCCAAGTTTTTTGAAGAGCCTGAAGATCCCAG CAGTAGGTCCTTCTTCTCAGAAATAATTTCATCCATATCCGATGTAAAATTCAGTCATAGTGGGCGGTAC ATGATGACCAGAGACTACCTGTCGGTGAAGGTGTGGGACCTCAACATGGAGAGCAGGCCGGTGGAGACCC ACCAGGTCCACGAGTACCTGCGCAGCAAGCTCTGCTCTCTCTATGAGAACGACTGCATCTTTGACAAGTT TGAGTGTTGCTGGAACGGTTCGGATAGCGCCATCATGACCGGGTCCTATAACAACTTCTTCAGGATGTTT GATAGAGACACGCGGAGGGATGTGACCCTGGAGGCCTCGAGAGAGAGCAGCAAACCGCGCGCCAGCCTCA AACCCCGGAAGGTGTGTACGGGGGGTAAGCGGAGGAAAGACGAGATCAGTGTGGACAGTCTGGACTTCAA CAAGAAGATCCTGCACACAGCCTGGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATAACTTG TACATATTCCAGGACAAAATCAACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001291310
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291310.1, NP_001278239.1
RefSeq Size	6791 bp
RefSeq ORF	867 bp
Locus ID	55844
UniProt ID	Q66LE6
Protein Families	Druggable Genome, Phosphatase
Protein Pathways	Tight junction
Gene Summary	B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an additional exon in the 5' region, and it thus differs in its 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) is shorter at the N-terminus, compared to isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.