DMC1 (NM_001278208) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	dJ199H16.1; DMC1H; LIM15
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278208, the custom clone sequence may differ by one or more nucleotides ATGAAGGAGGATCAAGTTGTGGCGGAAGAACCAGGATTCCAAGATGAAGAGGAATCTTTGTTTCAAGATA TTGACCTGTTACAGAAACATGGAATTAACGTGGCTGACATTAAGAAACTGAAATCAGTAGGAATCTGTAC CATCAAAGGTATACAGATGACAACAAGAAGAGCTCTATGCAATGTCAAAGGACTCTCAGAAGCCAAAGTA GACAAGATTAAAGAGGCAGCGAACAAACTAATTGAACCAGGATTCTTGACTGCATTTGAGTATAGTGAAA AGAGGAAAATGGTTTTCCATATCACCACCGGGAGCCAGGAATTTGATAAGTTACTAGGAGGTGGAATTGA AAGTATGGCAATTACAGAAGCTTTTGGAGAATTTCGTACTGGAAAAACCCAGCTTTCTCATACCCTCTGT GGTGAACATCAGATGGAGCTACTTGATTATGTAGCAGCAAAGTTCCATGAAGAAGCTGGCATCTTCAAGC TATTGATTATCGATTCAATAATGGCACTTTTTCGAGTGGATTTCAGTGGCCGTGGGGAGTTGGCCGAACG GCAGCAAAAATTGGCCCAGATGTTGTCACGACTCCAAAAAATCTCAGAAGAATATAACGTGGCTGTTTTT GTGACCAATCAAATGACTGCCGATCCAGGAGCAACTATGACCTTTCAGGCAGATCCCAAAAAACCCATTG GGGGACACATTCTGGCTCATGCTTCAACAACAAGAATAAGCTTGCGAAAGGGAAGAGGAGAGCTCAGAAT TGCCAAGATTTATGACAGTCCTGAGATGCCTGAAAATGAAGCCACCTTCGCAATAACTGCTGGAGGAATT GGGGATGCCAAGGAGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001278208
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278208.1, NP_001265137.1
RefSeq Size	2082 bp
RefSeq ORF	858 bp
Locus ID	11144
UniProt ID	Q14565
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the superfamily of recombinases (also called DNA strand-exchange proteins). Recombinases are important for repairing double-strand DNA breaks during mitosis and meiosis. This protein, which is evolutionarily conserved, is reported to be essential for meiotic homologous recombination and may thus play an important role in generating diversity of genetic information. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (2) differs in the 5' UTR and lacks two alternate in-frame exons in the coding region compared to variant 1. The resulting protein (isoform 2) lacks an internal portion compared to isoform 1. Variants 2 and 4 encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.