PSMG1 (NM_001261824) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C21LRP; DSCR2; LRPC21; PAC-1; PAC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001261824, the custom clone sequence may differ by one or more nucleotides ATGGCGGCCACGTTCTTCGGAGAGGTGGTGAAGGCGCCGTGCCGAGCTGGGACTGAGGACGAAGAGGAGG AGGAGGAGGGGCGGAGGGAGACGCCCGAGGACAGGGAGGTGCGTCTGCAGCTGGCGCGGAAGAGGGAAGT GCGGCTCCTTCGAAGACAAACAAAAACATCTTTGGAAGTTTCTTTGCTAGAAAAATATCCGTGCTCCAAG TTTATAATTGCTATAGGAAATAATGCAGTAGCATTTCTGTCATCATTTGTTATGAATTCAGGAGTCTGGG AGGAAGTTGGTTGTGCTAAACTCTGGAATGAATGGTGTAGAACAACAGACACTACACATCTGTCCTCCAC AGAGGCTTTTTGTGTGTTTTATCATCTAAAATCCAATCCCTCGTGCAGTTGCTATGTTGCAGAAGATCAA CAGTATCAGTGGCTGGAAAAGGTTTTTGGCTCTTGTCCAAGGAAGAACATGCAGATAACTATTCTCACAT GTCGACATGTTACCGATTATAAAACCTCAGAATCCACCGGCAGCCTTCCTTCTCCTTTCCTGAGAGCCCT AAAAACACAGAATTTCAAAGACTCGGCGTGTTGTCCATTGCTAGAACAACCGAATATAGTACACGACCTT CCTGCAGCAGTTCTAAGCTACTGTCAAGTATGGAAAATCCCAGCAATTCTGTACTTGTGTTATACTGATG TGATGAAATTAGACCTAATCACAGTGGAAGCTTTTAAGCCTATACTTTCTACCAGAAGCTTGAAGGGTTT GGTTAAGAATATTCCCCAAAGCACTGAGATACTAAAGAAATTGATGACAACAAATGAGATTCAGAGTAAC ATTTATACATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001261824
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261824.1, NP_001248753.1
RefSeq Size	1125 bp
RefSeq ORF	852 bp
Locus ID	8624
Gene Summary	Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

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