TSGA2 (RSPH1) (NM_001286506) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CT79; RSP44; RSPH10A; TSA2; TSGA2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286506, the custom clone sequence may differ by one or more nucleotides ATGTCGGACCTGGGCTCGGAGGAGTTGGAGGAGGAGGGAGAGAATGATATTGGGGGGATCTACAAATTTA AAAATGGTGCTCGATATATCGGAGAATATGTTAGAAATAAAAAGCACGGTCAAGGCACTTTTATATATCC AGATGGATCCAGATATGAAGGAGAGTGGGCAAATGACCTGCGGCACGGCCATGGCGTATACTACTACATC AATAATGACACCTACACTGGAGAGTGGTTTGCTCATCAAAGGCATGGGCAAGGCACCTATTTATACGCGG AGACGGGCAGTAAGTATGTTGGCACCTGGGTGAACGGACAGCAGGAGGGCACGGCCGAGCTCATTCACCT GAACCACAGGTACCAGGGCAAGTTCTTGAACAAAAATCCTGTTGGCCCTGGAAAGTATGTATTTGATGTT GGGTGTGAACAACATGGTGAATATCGTTTAACAGATATGGAAAGAGGAGAAGAGGAAGAGGAGGAAGAAT TAGTAACTGTTGTTCCAAAATGGAAAGCTACCCAAATCACTGAATTGGCCCTGTGGACACCAACTCTCCC CAAAAAGCCGACCTCTACGGATGGACCTGGCCAAGACGCTCCAGGAGCTGAGAGTGCAGGAGAACCCGGG GAGGAGGCCCAGGCTCTGCTGGAGGGCTTCGAGGGTGAGATGGACATGAGGCCTGGAGATGAAGATGCAG ACGTCCTCCGGGAAGAGAGCCGGGAGTATGACCAGGAGGAGTTCCGCTATGACATGGATGAGGGAAACAT TAATTCTGAAGAAGAAGAAACTAGACAGTCAGACCTCCAGGACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286506
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286506.1, NP_001273435.1
RefSeq Size	1331 bp
RefSeq ORF	816 bp
Locus ID	89765
UniProt ID	Q8WYR4
Gene Summary	This gene encodes a male meiotic metaphase chromosome-associated acidic protein. This gene is expressed in tissues with motile cilia or flagella, including the trachea, lungs, airway brushings, and testes. Mutations in this gene result in primary ciliary dyskinesia-24. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014] Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The resulting shorter isoform (2) lacks an internal protein segment compared to isoform 1.

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