ADPRH (NM_001291950) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARH1; hARH1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291950, the custom clone sequence may differ by one or more nucleotides ATGCAGCTGAAGCCGGGCAAGCCCAATGGCTGGAGGATTCCCTTCAACAGCCATGAGGGCGGCTGTGGGG CTGCCATGCGGGCCATGTGCATCGGTCTCAGGTTCCCACACCATAGCCAACTGGACACACTGATCCAAGT GAGCATCGAGAGTGGTCGGATGACCCACCACCACCCAACAGGCTACCTGGGGGCCCTTGCGTCTGCTCTT TTTACAGCCTATGCTGTGAATAGCAGACCACCCTTGCAGTGGGGAAAAGGACTGATGGAGCTGCTACCAG AAGCTAAAAAGTACATTGTCCAATCAGGCTACTTTGTAGAGGAAAATCTTCAACACTGGTCCTACTTCCA AACCAAATGGGAAAATTACCTAAAACTTAGAGGGATTTTGGATGGAGAATCAGCCCCTACCTTCCCTGAG TCTTTCGGTGTGAAGGAGAGGGATCAGTTCTACACCTCCCTGAGCTACTCTGGCTGGGGTGGCAGCAGTG GGCACGATGCCCCCATGATTGCCTACGATGCTGTTCTTGCTGCAGGAGACTCCTGGAAGGAGCTTGCCCA CCGAGCCTTTTTCCATGGTGGAGACAGTGATTCTACAGCTGCCATTGCTGGCTGCTGGTGGGGAGTTATG TATGGTTTTAAAGGAGTGAGTCCCTCCAACTATGAGAAACTAGAATACAGAAACCGGCTGGAAGAGACAG CTAGGGCTTTATATTCTCTCGGGTCAAAAGAAGACACTGTAATTTCCCTTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001291950
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291950.1, NP_001278879.1
RefSeq Size	3284 bp
RefSeq ORF	753 bp
Locus ID	141
UniProt ID	P54922
Gene Summary	The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014] Transcript Variant: This variant (3) lacks two exons, differs in the 5' UTR, and initiates translation at a downstream start codon compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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