DYRK4 (NM_001282286) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282286, the custom clone sequence may differ by one or more nucleotides ATGTGGAGCCTGGGCTGCATCACGGCGGAGTTGTACACGGGCTACCCCCTGTTCCCCGGGGAGAATGAGG TGGAGCAGCTGGCCTGCATCATGGAGGTGCTGGGTCTGCCGCCAGCCGGCTTCATTCAGACAGCCTCCAG GAGACAGACATTCTTTGATTCCAAAGGTTTTCCTAAAAATATAACCAACAACAGGGGGAAAAAAAGATAC CCAGATTCCAAGGACCTCACGATGGTGCTGAAAACCTATGACACCAGCTTCCTGGACTTTCTCAGAAGGT GTTTGGTATGGGAACCTTCTCTTCGCATGACCCCGGACCAGGCCCTCAAGCATGCTTGGATTCATCAGTC TCGGAACCTCAAGCCACAGCCCAGGCCCCAGACCCTGAGGAAATCCAATTCCTTTTTCCCCTCTGAGACA AGGAAGGACAAGGTTCAAGGCTGTCATCACTCGAGCAGAAAAGATGAGATCACCAAAGAGACTACAGAGA AAACAAAAGATAGCCCCACGAAGCATGTTCAGCATTCAGGTGATCAGCAGGACTGTCTCCAGCACGGAGC TGACACTGTTCAGCTGCCTCAACTGGTAGACGCTCCCAAGAAGTCAGAGGCAGCTGTCGGGGCGGAGGTG TCCATGACCTCCCCAGGACAGAGCAAAAACTTCTCCCTCAAGAACACAAACGTTTTACCCCCTATTGTAT GA
Restriction Sites	SgfI-MluI
ACCN	NM_001282286
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282286.1, NP_001269215.1
RefSeq Size	965 bp
RefSeq ORF	702 bp
Locus ID	8798
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (3) differs in the 5' UTR, represents the use of an alternate promoter which results in translation initiation at a downstream AUG, and uses an alternate in-frame splice site in the 3' terminal exon compared to variant 1. The resulting isoform (3) has a shorter N-terminus compared to isoform 1.

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